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  9. Петлевая LAMP амплификация нуклеиновых кислот. II. Детекция коронавируса SARS-CoV-2 с помощью различных вариантов обратно-транскрипционной петлевой амплификации

Петлевая LAMP амплификация нуклеиновых кислот. II. Детекция коронавируса SARS-CoV-2 с помощью различных вариантов обратно-транскрипционной петлевой амплификации

Год: 2023

Страницы: 272-290

Номер: Том 15, № 4

Тип: научная статья

DOI: https://doi.org/10.31301/2221-6197.bmcs.2023-24

Рубрика: Статьи

Авторы: Гарафутдинов Равиль Ринатович, Ахметзянова Лиана Ульфатовна, Сахабутдинова Ассоль Рафиковна, Чемерис Дмитрий Алексеевич, Чемерис Алексей Викторович, Губайдуллин Ирек Марсович

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Аннотация:

Несмотря на то, что «золотым стандартом» для диагностики нового коронавируса SARS-CoV-2 остается ОТ- ПЦР, все активнее начинает применяться обратно-транскрипционная петлевая изотермическая амплификация (RT-LAMP). Она имеет определенные преимущества ввиду изотермичности процесса и относительной простоты детекции лампликонов. Это позволяет позиционировать эту реакцию как проводимую по месту лечения, в том числе применительно к выявлению SARS-CoV-2. Способы детекции результатов любой разновидности LAMP можно подразделить на специфичные (прямые), рассчитанные на использование гибридизационных зондов, и косвенные, при которых контролируется общее накопление лампликонов или побочных продуктов реакции (пирофосфата и протонов). Для обнаружения генетического материала SARS-CoV-2 в клинических образцах с помощью RT-LAMP предложено большое количество вариантов, основанных на разных способах детекции. Важным является обеспечение высокой специфичности любых вариантов LAMP, которая зависит от качества подбора праймеров, осуществляемого с помощью компьютерных программ.

Ключевые слова:

коронавирус, SARS-CoV-2, РНК, RT-LAMP, лампликон, , изотермическая амплификация, обратно-транскрипционная петлевая амплификация, детекция, программа дизайна праймеров

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