Biomics

For the first time, a thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus was isolated in 1976, but its use in PCR began only more than ten years later, despite the fact that PCR itself had been developed a couple of years earlier. By the time this enzyme began to be used in PCR instead of the Klenow fragment of E.coli DNA polymerase I, DNA polymerases from related eubacteria T. thermophilus, T. flavus, and T. ruber, as well as DNA polymerases from thermophilic archaea Sulfolobus acidocaldarius, S. solfataricus, and Methanobacterium thermoautotrophicum, were already known, in addition to Taq-polymerase. However, many years passed before some of them were used in PCR.

DNA polymerase, Klenow fragment of DNA polymerase I E. coli, thermostable DNA polymerase, DNA, PCR, Thermus aquaticus

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