Comparison of genetic markers of resistance and phenotypic profile of antimicrobial resistance in clinical isolates of Escherichia coli and Klebsiella pneumoniae.
Year: 2024
Pages: 369-380
Number: Volume 16, issue 4
Type: scientific article
DOI: https://doi.org/10.31301/2221-6197.bmcs.2024-26
Topic: Articles
Authors: Yadykova L.L, Zykova D.A., Bayazitova L.T., Trizna E.Y.
Summary:
Methods. The isolates of E. coli and K. pneumoniae were obtained from KRIEM of Rospotrebnadzor. The sensitivity of clinical isolates to antibiotics was assessed using the disk diffusion method according to the recommendations of the International Association of Microorganisms of the Russian Federation (IACMAC). The presence of genetic markers of resistance in clinical isolates was determined using the polymerase chain reaction.
Results. Clinical isolates of E. coli and K. pneumoniae were resistant to different classes of antibiotics, and often one isolate was resistant to drugs of up to 3 classes. Meropenem was effective against the most of isolates. Only in some phenotypically resistant isolates the presence of common genetic markers mexB, mexD, ampC, aac(3)-IIa, aphA, aadB associated with antibiotic resistance was confirmed. This may call into question the relevance of using these markers to identify resistance genes.
Keywords:
antibiotics. antibiotic resistance, Enterobacteriaceae, E. coli, K. pneumoniae
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