eISSN: 2221-6197 DOI: 10.31301/2221-6197

Analysis of quality indicators of vegetable jelly

Year: 2024

Pages: 381-390

Number: Volume 16, issue 4

Type: scientific article

Summary:

Bacteria of Enterobacteriaceae family are among the most common pathogens worldwide. The massive inappropriate use of antibiotics has led to the development and spread of multidrug resistance in them. Various mechanisms of resistance are now known and may be determined by antibiotic resistance genes. However, data of the spread of resistance genes among bacteria in the Russian Federation are scattered or absent. 
Methods. The isolates of E. coli and K. pneumoniae were obtained from KRIEM of Rospotrebnadzor. The sensitivity of clinical isolates to antibiotics was assessed using the disk diffusion method according to the recommendations of the International Association of Microorganisms of the Russian Federation (IACMAC). The presence of genetic markers of resistance in clinical isolates was determined using the polymerase chain reaction.
Results. Clinical isolates of E. coli and K. pneumoniae were resistant to different classes of antibiotics, and often one isolate was resistant to drugs of up to 3 classes. Meropenem was effective against the most of isolates. Only in some phenotypically resistant isolates the presence of common genetic markers mexB, mexD, ampC, aac(3)-IIa, aphA, aadB associated with antibiotic resistance was confirmed. This may call into question the relevance of using these markers to identify resistance genes.
Conclusion. Monitoring of the resistance genes spread is necessary to optimize the use of antibacterial drugs, taking into account the risks of horizontal transfer of genetic markers and to reduce the rate of their spread among pathogenic and opportunistic microorganisms. 

Keywords:

fruit and vegetable juices, jellies, physicochemical parameters, microbiological parameters, organoleptic parameters

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eISSN: 2221-6197 DOI: 10.31301/2221-6197