Biomics

The principle and features of multiplex PCR are considered. Attention is focused on the advantages of the reaction: reduction of time, financial and labor costs during parallel analyses, as well as the possibility of analyzing a small amount of genetic material. It is noted that high-quality primers are the main factor ensuring the specificity of multiplex PCR, but other components of the reaction mixture should also be optimized. The difference between multiplex PCR and multitemplate PCR is demonstrated. Data on methods for increasing the efficiency of multiplex PCR are presented, among which "hot start" and modified thermal cycling protocols are emphasized. Attention is paid to the history of multiplex PCR and the terminology used. 

PCR, multiplex PCR, primers

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