Biomics

Orthologs of β-1,3-glycanase (BG) of Arabidopsis thaliana L. were identified in flax Linum usitatissimum L. in silico. They corresponded to 57 sequences grouped into 27 orthologous groups. Protein domains characteristics of β-1,3-glucanases were predicted for 22 main orthologs showing changes in expression at the gene level during the development of the fiber (individual cell) in normal conditions (different stages), and an analysis of temporal (8 h, 24 h, 96 h after tilt of stem) and spatial (upper (PUL) and lower (OPP) sides of the plant stem) expression profiles during gravity response was performed. Most of the analyzed BG showed maximum expression in the apical meristem. In response to gravistimulation, a change in expression was detected in 11 BG genes. Of these, 7 genes showed increased expression in the fibers of both sides of the stem (Lus10015151, Lus10040461, Lus10033244), of PUL-side (Lus10019801, Lus10039095 and Lus10032562) and of OPP-side (Lus10024535). A decrease in expression during gravity response was revealed in genes Lus10011482, Lus10002295, Lus10043075 and Lus10038099. For Lus10011482 and Lus10043075, activity was detected on both sides of the stem 8, 24 and 96 hours after the plants were tilted. With gravity response, the value of gene expression of the main orthologs changed towards earlier stage of fiber development and was often confirmed by a change in the expression of all genes of the corresponding orthologous group. It is suggested that the corresponding β-1,3-glucanases are localized in the cell wall and modified to manifest the "muscular" functions of the fibers. This study provides a basis for elucidating the functional characteristics of β-1,3-glucanases during fiber development and during gravity response. 

gravity response, flax, fiber, tertiary cell wall, β-1,3-glucanase, gene expression

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