Biomics

Single nucleotide substitutions (SNPs) account for the largest share of DNA polymorphism in virtually any organism, including humans, and have a significant impact on their life status. Detection of already known polymorphic nucleotides (SNP typing) is of great importance, which explains the extremely wide variety of existing methods for SNP analysis. One of the most widely used approaches is allele-specific PCR (ASPCR) with primers characterized by differences in structure and the resulting ability to discriminate polymorphic nucleotides in DNA. AS-PCR is implemented in a variety of variants (about fifty), which are briefly considered in this review.

DNA, single-nucleotide polymorphism, SNP, PCR, allele-specific PCR, ASPCR, SNP typing

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