Разнообразие методов детекции полиморфных нуклеотидов в известных снипах. III. Аллель-специфичная ПЦР
26.07.2022
Авторы:
Название:
Разнообразие методов детекции полиморфных нуклеотидов в известных снипах. III. Аллель-специфичная ПЦР
Страницы:
32-51
Замены одиночных нуклеотидов (SNP) составляют наибольшую долю полиморфизма ДНК практически любых организмов, включая человека, и оказывают заметное влияние на их жизненный статус. Детекция уже известных полиморфных нуклеотидов (SNP-типирование) приобретает важное значение, чем объясняется чрезвычайно большое разнообразие существующих методов анализа SNP. Одним из наиболее широко применяемых подходов является аллель-специфичная ПЦР (АС-ПЦР) с праймерами, характеризующимися различиями в строении и обусловленной этим способностью дискриминировать полиморфные нуклеотиды в ДНК. АС-ПЦР реализована во множестве вариантов (около полусотни), кратко рассмотренных в данном обзоре.
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