The influence of temperature on the multimeric products formation by strand-displacement DNA polymerases in the conditions of isothermal amplification
Year: 2020
Pages: 469-474
Number: Volume 13, issue 4
DOI: https://doi.org/10.31301/2221-6197.bmcs.2020-40
Topic: Article
Authors: Gilvanov A.R.!, Sakhabutdinova A.R.!, Garafutdinov R.R.!
Summary:
The nucleic acids amplification is one of the key methods for molecular biology research and clinical diagnostics. The isothermal amplification methods, for example rolling circle amplification, are a good alternative for a widely spread polymerase chain reaction. Strand-displacement DNA polymerases are required for isothermal amplification. In this work, we studied the influence of temperature on the formation of specific and non-specific amplification products by 9°Nm, Vent exo- , Hemo KlenTaq DNA polymerases during rolling circle amplification. The temperature values for the most effective formation of non-specific products and specific concatemeric products were determined. The obtained data will allow the development of more specific isothermal amplification methods with DNA polymerases used.
Keywords:
isothermal DNA amplification; multimerization; DNA polymerases; strand-displacing activity
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