Biomics

Five strains of R. leguminosarum isolated from nodules of pea plants were studied in the work. The highest value of kinematic viscosity was observed in the growth medium of bacteria R. leguminosarum V30 and corresponded to a value of 30 mm²/s. Extraction with ethanol from the culture liquid of the bacteria R. leguminosarum V30 yielded a gelatinous, transparent precipitate of polysaccharides. After drying the gel, a light water-soluble powder was obtained. The studies made it possible to isolate a new strain of bacteria with a high biotechnological potential. The growth R. leguminosarum V30 on a cheap substrate makes it possible to obtain a large volume of culture liquid with high kinematic viscosity in 5-6 days. Extraction of the valuable metabolite R. leguminosarum V30 is carried out in a simple way and allows to isolate up to 5 g of a polysaccharide preparation from 1 liter of culture liquid.

batch cultivation; kinematic viscosity, exopolysaccharides; Rhizobium

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