Biomics

Transgenic tobacco plants expressing gene GORL of east goat's-rue Galega orientalis lectin under control of the cauliflower mosaic virus 35S promotor were obtained. Research of adhesion on roots of these plants fluorescently labeled bacteria Rhizobium galegae (goat’s-rue microsymbiont) showed that on transgenic tobacco roots with lectin gene GORL were absorbed in 4 times more bacteria R. galegae in comparison with control non-transgenic plants. The received results testify to participation of the GORL lectin in attachment of bacteria to a surface of transgenic plant roots, however, this interaction has low-specific character. Nevertheless, the similar strategy based on use of legume plants lectin as transgenes for initiation of recognition processes can be used further for receiving new associative systems economically valuable the non-symbiotic plants and bacteria species possessing various useful properties.

goat’s-rue, Galega orientalis, lectin, transgenic plants, tobacco, root colonization, rhizobia, legume-rhizobial symbiosis

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