Biomics

Immunohystochemical localization of lipid transfer proteins (LTPs) in the central and basal root zones of pea plants showed their presence on the periphery of cells mainly located in the phloem. Addition of NaCI (50mM) to the nutrient solution resulted in increased level of LTPs immunostaining in the phloem. The deposition of suberin in endoderma (Casparian bands) with Sudan III was more intensive under salt stress and co-located with enhanced LTP localization. These data confirm the involvement of pea LTPs in the suberin deposition. Salt stress led to the increase in ABA immunostaining in root cells but its localization was not coincident with LTPs. Thus the assumption of the direct ABA influence on increased LTPs in pea root cells under salinity was not confirmed. The role of LTPs in plants is discussed.

pea, lipid transfer proteins, ABA, immunolocalization, suberin, salt stress

1. Abdelkader, A.B., Mazliak P. Echanges de lipides entre mitochondries, microsomes et surnageant cytoplasmique de cellules de pomme de terre ou de chou-fleur. J. Biochem. 1970. V. 15. P. 250–262.
2. Akhiyarova G.R., Fricke W., Veselov D.S., Kudoyarova G.R., Veselov S.Yu. ABA accumulation and distribution during the leaf tissues shows its role stomatal conductance regulation under short – term salinity. Citologiya. 2006. T. 48. № 11. S. 918–923.
3. Bogdanov I.V., Finkina E.I., Balandin S.V., Melnikova D.N., Stukacheva E.A., Ovchinnikova T.V. Structural and functional characterization of recombinant isoforms of the lentil lipid transfer protein. Acta Naturae. 2015. V. 7. P. 65-73.
4. Bogdanov I.V., Shenkarev Z.O., Finkina E.I., Melnikova D.N., Rumynskiy E.I., Arseniev A.S., Ovchinnikova T.V. A novel lipid transfer protein from the pea Pisum sativum: isolation, recombinant expression, solution structure, antifungal activity, lipid binding, and allergenic properties. BMC Plant Biol. 2016. V. 16. P. 107-124. DOI: 10.1186/s12870-016-0792-6
5. Cameron K.D., Teece M.A., Smart L.B. Increased accumulation of cuticular wax and expression of lipid transfer protein in response to periodic drying events in leaves of tree tobacco. Plant Physiol. V. 140. Р. 176–183. DOI: 10.1104/pp.105.069724
6. Carella P., Merl - Pham J., Wilson D.C., Dey S., Hauck S.M., Vlot A.C. Cameron R.K. Comparative proteomics analysis of phloem exudates collected during the induction of systemic acquired resistance. Plant Physiol. V.171. P.1495-510. DOI: 10.1104//pp.16.00269
7. Cheng C.S., Samuel D., Liu Y.J., Shyu J. C., Lai S.M., Lin K.F., Lyu P.C. Binding mechanism of nonspecific lipid transfer proteins and their role in plant defense. 2004. V. 43. P. 13628–13636. DOI: 10.1021/bi048873j
8. Douliez J.P., Michon T., Marion D. Steady-state tyrosine fluorescence to study the lipid-binding properties of a wheat non-specific lipid-transfer protein (nsLTP1). Biophys. Acta. 2000. V. 1467. P. 65–72. DOI: 10.1016/S0005-2736(00)00197-8
9. Edstam M.M., Edqvist J. Involvement of GPI-anchored lipid transfer proteins in the development of seed coats and pollen in Arabidopsis thaliana. Physiol Plant. 2014. V. 152(1). Р. 32–42. DOI: 10.1111/ppl.12156
10. Finkina E.I., Melnikova D.N., Bogdanov I.V., Ovchinnikova T.V. Belki sistemi vrojdennogo immuniteta rastenii, osuschestvlyayuschie transport lipidov: struktura, funkcii i prakticheskoe primenenie. Acta Naturae. 2016. Т.8. № 2(29). S. 20-36.
11. Furst G.G. Metodi anatomo - gistohimicheskogo issledovaniya rastitelnih tkanei. M.: Nauka, 1979. 155 s.
12. Guelette B.S., Benning U.F., Hoffman-Benning S. Identification of lipids and lipid binding proteins in phloem exudate from Arabidopsis thaliana. Exp. Bot. 2012. V. 63. P. 3603-3616. DOI: 10.1093/jxb/ers028
13. Hoh F., Pons J.L., Gautier M.F., de Lamotte F., Dumas C. Structure of a liganded type 2 non-specific lipid transfer protein from wheat and the molecular basis of lipid binding. Acta Crystallogr. D. Biol. Crystallogr. V.61. P.397–406. DOI: 10.1107/S09074449050004174
14. Kader J.C. Lipid-transfer proteins in plants. Rev. Plant. Physiol. Plant. Mol. Biol. 1996. V. 47. P. 627–654. DOI: 10.1146/annurev.arplant.47.1.627
15. Kim T.H., Kim M.C., Park J.H. Differential expression of rice lipid transfer protein gene (LTP) classes in response to abscisic acid, salt, salicylic acid, and the fungal pathogen Magnaporthe grisea. Plant Biol. 2006. V. 49. P. 371-375. DOI: 10.1007/BF03178814
16. Lee S.B., Go Y.S., Bae H.J., Park J.H., Cho S.H., Cho H.J., Lee D.S., Park O.K., Hwang I., Suh M.C. Disruption of glycosylphosphatidylinositol-anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglobules, and enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola. Plant Physiol. V. 150. № 1. Р. 42–54. DOI: 10.1104/pp.109.137745
17. Pato C., Borgne M., Baut G., Papec P., Marion D., Douliez J.P. Potential application of plant lipid transfer proteins for drug delivery. Pharmacol. 2001. V. 62. P. 555–560. DOI: 10.1016/S0006-2952(01)00708-0
18. Perumalla C.J., Peterson C.A. Deposition of Casparian bands and suberin lamellae in the exodermis and endodermis of young corn and onion roots. J. Bot. 1986. V. 64. P. 1873-1878.
19. Samuel D., Liu Y.J., Cheng C.S., Lyu P.C. Solution structure of plant nonspecific lipid transfer protein-2 from rice (Oryza sativa). Biol. Chem. 2002. V. 277. P. 35267–35273. DOI: 10.1074/jbc.M203113200
20. Simorre J., Caille A., Dominique M., Didier M.,.Ptak M. Two- and three-dimensional 1H NMR studies of a wheat phospholipid transfer protein: sequential resonance assignments and secondary structure. 1991. V. 30. P. 11600–11608. DOI: 10.1021/bi00113a016
21. Tassin S., Broekaert W. F., Marion D., Acland D.P., Ptak M., VovelleF., Sodano P. Solution structure of ace-amp1, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins. 1998. V. 37. P. 3623–3637. DOI: 10.1021/bi9723515
22. Tsuboi S., Osafune T., Tsugeki R., Nishimura M., Yamada M. Nonspecific lipid transfer protein in castor bean cotyledon cells: subcellular localization and a possible role in lipid metabolism. Biochem. 1992. V. 3. P. 500–508.
23. Veselov S.Yu. Ispolzovanie antitel dlya kolichestvennogo opredeleniya, ochistki i lokalizacii regulyatorov rosta rastenii. Ufa: Bashkirskii universitet, 138 s.
24. Veselov D.S., Sharipova G.V., Veselov S.Yu., Kudoyarova G.R. The effects of NaCl treatment on water relations, growth and ABA content in barley cultivars differing in drought tolerance. Plant Growth Regul. 2008. V. 27. P. 380–386. DOI: 10.1007/s00344-008-9064-5