Biomics

Inoculation of A. niger AM1 in medium containing just two sources of phosphorus (phosphate and white phosphorus) demonstrated that P₄ in a concentration of 0.2% does not exhibit toxic properties in relation to this microorganism. In the presence of white phosphorus it grows at the same rate as in the absence thereof. Comparison of sequences of ribosomal genes of fungi with those in the GenBank database, allowed us to identify this microorganism as a new strain of Aspergillus niger, which we assigned a designation A. niger АМ1. To compare the conversion of white phosphorus in the presence and absence of microbial culture A. niger AM1 were sown in a medium with white phosphorus, sterilized with acetone. The sowing was performed in a test, the control medium with white phosphorus left sterile. In control media no growth was detected in 117 days. This indicates that the sterilization of P₄ weighs with acetone is effective.

Biodegradation, detoxication, white phosphorus, toxicity, metabolism, Aspergillus niger АМ1, culture medium, sterilization

1. Engelking L.R. Textbook of Veterinary Physiological Chemistry (Third Edition). Academic Press. 2015. 786 p. Chapter 85 – Bicarbonate, Phosphate, and Ammonia Buffer Systems. P. 549-554.
2. Hausinger R.P. New insights into acetone metabolism. J Bacteriol. 2007. Vol.189. No.3. P.671-673.
3. Kassim E.-S.A. Cellulase enzyme from Aspergillus niger. Microbiol. Immunol. 1982. Vol. 26. No.6. 449- 454.
4. Mindubaev A.Z. Kto s"el polietilen? Nauka i Zhizn. 2018. No. 4. P. 32-38. [Who ate the polyethylene? - In Russian].
5. Mindubaev A.Z., Voloshina A.D, Kulik N.V., Saparmyradov K.A., Minzanova S.T, Mironova L.G, Khayarov Kh.R. Resistance to white phosphorus of fungi and streptomycetes. Biomics. 2018. V.10(2). P. 214- 219. (In Russian)
6. Mindubaev A.Z., Voloshina A.D., Gorbachuk E.V., Validov S.Z., Kulik N.V., Alimova F.K., Minzanova S.T., Mironova L.G., Belostotskiy D.E., Saparmyradov K.A., Tukhbatova R.I., Yakhvarov D.G. Adaptation of microorganisms to white phosphorus as a result of directed selection. Genetic identification of sustainable Aspergillus and metabolic profiling of Streptomyces A8. Butlerov Communications. 2015. V. 44(12). P. 1- 28. (In Russian).
7. Mindubaev A.Z., Voloshina A.D., Validov S.Z., Kulik N.V., Minzanova S.T., Mironova L.G., Yakhvarov D.G., Akkizov A.Y.. Aspergillus niger AM1 culture growth in medium with two phosphorus sources. The validity of the definition "biodegradation" with respect to white phosphorus. Butlerov Communications. 2016. V. 46(5). P. 1-20. (In Russian).
8. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual, Volume 1, 2, 3. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, New York. 2001. 2344 p.