Biomics

The possibility of the analysis of the bacterial cells by the example of Azospirillum brasilense soil microorganisms of the strain Sp7 due to their infection by the specific antibodies was studied by using an acoustic sensor directly in a suspension with the different initial electrical conductivity. The sensor represented a two-channel delay line based on a piezoelectric lithium niobate plate with propagating the shear-horizontal acoustic wave of zero order. One channel of delay line was electrically free, and the second one was electrically shorted. The liquid container was placed on the plate in such a way that the suspension was in contact with the surface of the plate and covered both channels. The analysis was carried out by measuring the time dependence of the phase and insertion loss of the sensor output signal at a fixed frequency before and after the biological interaction of the microbial cells with specific antibodies. The conductivity of the buffer solution was changed from 2.2 to 10 μS/cm, and the minimum detectable concentration of the microbial cells was equal to ~ 10⁴ cells/ml.

Azospirillum brasilense, acoustic sensor, nitrogen fixation, ecological microbiology

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