Biomics

Structure and properties of the surface antigens of rhizosphere bacteria is the major scope of research of the IBPPM RAS Laboratory of Immunochemistry. We use antibodies raised to bacterial protein and carbohydrate structures – the surface structures of 27 strains belonging to 11 species of 7 genera of associative bacteria – that play a key part in associative plant-microbe interactions in the plant rhizosphere. While using a variety of Azospirillum strains, we showed that their O antigens were structurally and antigenically heterogeneous and several Azospirillum strains lacked an individual capsular antigen. We developed a test system for Azospirillum serotyping with the use of 74 Azospirillum strains. Glycosylated flagellin of the polar flagellum from A. brasilense type strain Sp7 was shown to contain several polysaccharide chains that are immunochemically identical to the lipopolysaccharide of this bacterium. Enzyme-linked immunosorbent assay was used as the basis for analyzing the occurrence of Azospirillum in soil samples, allowing us to assess the population dynamics of soil-introduced bacteria and to detect Azospirillum antigens in six types of soil in Saratov Oblast during the vegetative season. The strains’ abilities to colonize plant roots were assessed. Plants’ morphometric and cytological variables were used to detect promoting effect of bacteria and their isolated surface components. We showed the contribution of Azospirillum lipopolysaccharide, flagellin, and proteinaceous pilus-like structures to the bacterial colonization of plants and to the induction of growth promotion. We are now isolating and identifying new rhizospheric strains that engage in symbiosis with plants (members of the genera Enterobacter, Ochrobactrum, Ensifer, Acinetobacter, Kocuria, etc.), with assessment of their growth-promoting potential.

Azospirillum; plant-microbe interactions; associative symbiosis; carbohydrate and protein antigens; pilus-like structures; bacterial colonization; plant growth promoting effects

1. Belyakov A.Ye., Burygin G.L., Arbatsky N.P., Shashkov A.S., Selivanov N.Y., Matora L.Y., Knirel Y.A., Shchyogolev S.Y. Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp Carbohydr. Res. 2012. V. 361. P. 127-132. DOI: 10.1016/j.carres.2012.08.019
2. Bogatyrev V.A. Dykman L.A., Matora L.Yu., Schwartsburd B.I. The serotyping of Azospirillum spp. by cell-gold immunoblotting. FEMS Microbiol. Lett. 1992. Vol. 96. P. 115-118. DOI: 10.1111/j.1574-6968.1992.tbx
3. Burygin G.L., Popova I.A., Kargapolova K.Yu., Tkachenko O.V., Matora L.Yu., Shchyogolev S.Yu. A bacterial isolate from the rhizosphere of potato (Solanum tuberosum L.) identified as Ochrobactrum lupini IPA2. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology]. 2017. Vol. 52, № 1. P. 105-115. DOI: 10.15389/agrobiology.2017.1.105rus (In Russian - Бурыгин Г.Л., Попова И.А., Каргаполова К.Ю., Ткаченко О.В., Матора Л.Ю., Щеголев С.Ю. Бактериальный изолят из ризосферы картофеля (Solanum tuberosum L.), идентифицированный как Ochrobactrum lupini IPA7.2. Сельхозяйственная биология. 2017. Т. 52, №1. С. 105-115.
4. Dykman L.A., Staroverov S.A., Bogatyrev V.A., Shchegolev S.Yu. Gold nanoparticles as an antigen carrier and an adjuvant. New-York: Nova Sci. Publ., 2010. 54 p.
5. Dykman L.A., Khlebtsov N.G. Gold Nanoparticles in Biomedical Applications. Boca Raton: CRC Press, 2017. 332 p.
6. Evseeva N.V., Matora L.Yu., Burygin G.L., Dmitrienko V.V., Shchyogolev S.Yu. Effect of Azospirillum brasilense Sp245 lipopolysaccharide on the functional activity of wheat root meristematic cells. Plant Soil. V. 346. P. 181-188. DOI: 10.1007/s11104-011-0808-9
7. Krasov A.I., Popova I.A., Filip’echeva Y.A., BuryginG.L., Matora L.Y. Application of enzyme immunoassay for detection of the nitrogen-fixing bacteria of the genus Azospirillum in soil suspensions. (Microbiologiya) 2009. V. 78. P. 598-602. DOI: 10.1134/S0026261709050117 (In Russian - Красов А.И., Попова И.А., Филипьечева Ю.А., Бурыгин Г.Л., Матора Л.Ю. Применение иммуноферментного анализа для выявления азотфиксирующих бактерий рода Azospirillum в почвенных суспензиях. Микробиология. 2009. Т.78, № 5. С. 662-666.
8. Matora L.Yu., Shvartsburd B.I., Shchegolev S.Yu. Immunochemical analysis of O-specific polysaccharides of soil nitrogen-fixing bacteria Azospirillum brasilense. Mikrobiologiya. 1998. V. 67. P. 815-820. DOI: 10.1023/A:1015146104397 (In Russian - Матора Л.Ю., Шварцбурд Б.И., Щеголев С.Ю. Иммунохимический анализ О-специфических полисахаридов почвенных азотфиксирующих бактерий Azospirillum brasilense. Микробиология. Т. 67(6). С. 815-820.
9. Matora L.Yu., Shchegolev S.Yu. Antigenic identity of the capsule lipopolysaccharides, exopolysaccharides, and O-specific polysaccharides in Azospirillum brasilense. Microbiology (Microbiologiya.). V. 71. P. 178-181. doi: 10.1023/A:1015146104397 (In Russian - Матора Л.Ю., Щеголев С.Ю. Антигенная идентичность липополисахаридов, капсулы и экзополисахаридов Azospirillum brasilense. Микробиология. 2002. Т. 71, №2. С. 211-214.
10. Matora L.Yu, Burygin G.L., Shchyogolev S.Yu. Study of immunochemical heterogeneity of Azospirillum brasilense Microbiology (Microbiologiya.). 2008. V. 77. P. 166-170.] DOI: 10.1134/S0026261708020070 (In Russian - Матора Л.Ю., Бурыгин Г.Л., Щеголев С.Ю. Исследование иммунохимической гетерогенности липополисахаридов Azospirillum brasilense. Микробиология. 2008. Т. 77, № 2. С. 196-200.
11. Sigida E.N., Fedonenko Y.P., Zdorovenko E.L., Burygin G.L., Konnova S.A., Ignatov V.V. Characterization of the lipopolysaccharides of serogroup II Azospirillum Microbiology (Microbiologiya). 2014. V. 83. P. 326-334. DOI: 10.7868/S0026365614040156 (In Russian - Сигида Е.Н., Федоненко Ю.П., Здоровенко Э. Л., Бурыгин Г.Л., Коннова С.А., Игнатов В.В. Характеристика липополисахаридов бактерий рода Azospirillum, отнесенных к серогруппе II. Микробиология. 2014. Т. 83. №. 4. С. 416-416.
12. Shirokov A.A., Krasov A.I., Selivanov N.Y., Burygin G.L., Shchyogolev S.Y., Matora L.Y. Immunochemical detection of Azospirilla in soil with genus-specific antibodies. Microbiology (Microbiologiya). V. 84. P. 263-267. DOI: 10.7868/S0026365615020135 (In Russian - Широков А.А., Красов А.И., Селиванов Н.Ю., Бурыгин Г.Л., Щеголев С.Ю., Матора Л.Ю. Иммунохимическое выявление бактерий рода Azospirillum в почве с помощью родоспецифичных антител. Микробиология. 2015. Т. 84, № 2. С. 244-249.
13. Shchyogolev Yu. On prokaryote systematics: topical problems and ways out of the crisis. Yu.A. Ovchinnikov bulletin of biotechnology and physical and chemical biology. 2018. V.14(1). P.5-14. (In Russian - Щеголев С.Ю. О систематике прокариот: актуальные проблемы и пути выхода из кризиса. Вестник физико-химической биологии и биотехнологии. 2018. Т. 14(1). C.5-14.
14. Shchyogolev S.Yu., Burygin G.L. Application of modern approaches to genetic systematics of prokaryotes in taxonomic studies of rhizosphere microflora. Biomics. 2018. V.10(2). P.156-160. DOI: 31301/2221-6197.bmcs.2018-20 (In Russian - Щеголев С.Ю., Бурыгин Г.Л. Опыт применения современных подходов к геносистематике прокариот в таксономических исследованиях ризосферной микрофлоры. Биомика. 2018. Т. 10(2). С.156-160. DOI: 10.31301/2221-6197.bmcs.2018-21