Biomics

Among transgenic tomato plants with genes encoding chitin-binding proteins from Amaranthus caudatus L. (ac) and Amaranthus retroflexus L. (RS intron-Shir) as well as hevein-like antimicrobial peptides from Stellaria media L. (SmAMP1 and SmAMP2) were identified lines with normal and parthenocarpic fruits. The fruit structure in transgenic plants is compared. Significant differences between control and transgenic plants with a normal and abnormal phenotype were found in the quantitative ratio of diploid and polyploid cells in the pericarp of the fruit. Possible reasons for the formation of parthenocarpic fruits in transgenic plants are discussed.

tomato (Solanum lycopersicum L.), Agrobacterium-mediated transformation, parthenocarpy, pericarp cell ploidy

1. Bourdon M., Coriton O., Pirrello J., Cheniclet C., Brown S.C., Poujol C., Chevalier C., Renaudin J.P., Frangne N. In Planta quantification of endoreduplication using fluorescent in situ hybridization (FISH) // J. Plant Sci. 2011. V. 66. № 6. P. 1089–1099. DOI: 10.1111/j.1365-313X.2011.04568.x
2. Ficcadenti N., Sestili S., Pandolfini T., Cirillo C., Rotino G.L., Spena A. Genetic engineering of parthenocarpic fruit development in tomato // Mol. Breeding. 1999. V. 5. № 5. P. 463–470. DOI: 10.1023/A:100966540
3. de Jong M., Mariani C., Vriezen W.H. The role of auxin and gibberellin in tomato fruit set // J. Exp. Bot. 2009. V. 60. № 5. P. 1523–1532. DOI: 10.1093/jxb/erp094
4. García-Hurtado N., Carrera E., Ruiz-Rivero O., López-Gresa M.P., Hedden P., Gong F., García-Martínez J.L. The characterization of transgenic tomato overexpressing gibberellin 20-oxidase reveals induction of parthenocarpic fruit growth, higher yield, and alteration of the gibberellin biosynthetic pathway // J. Exp. Bot. 2012. V. 63. № 16. P. 5803-5813. DOI:10.1093/jxb/ers229
5. Gorquet B., van Heusden A.W., Lindhout P. Parthenocarpic fruit development in tomato // Plant Biol. 2005. V. 7. № 2. P. 131–139. DOI: 10.1055/s-2005-837494
6. Khaliluev M.R., Kharchenko P.N., Dolgov S.V. Genetic Transformation of Tomato (Solanum lycopersicum L.) by protective chitin binding protein and antimicrobial peptide genes // Izv. TSKhA. 2010. № 6. P. 75–83. [In Russian]. Халилуев М.Р., Харченко П.Н., Долгов С.В. Генетическая трансформация томата (Solanum lycopersicum L.) генами защитных хитин-связывающих белков и антимикробных пептидов // Известия ТСХА. 2010. № 6. С. 75–83.
7. Khaliluev M.R. , Chaban I.A., Kononenko N.V., Baranova E.N., Dolgov S.V., Kharchenko P.N., Polyakov V.Yu. Abnormal floral meristem development in transgenic tomato plants do not depend on the expression of genes encoding defense-related PR-proteins and antimicrobial peptides // Russ. J. Dev. Biol. 2014. V. 45. № 1. P. 22-33. DOI: 10.1134/S1062360414010044
8. S¸en A. Oxidative stress studies in plant tissue culture. In Antioxidant Enzyme. Rijeka, Croatia: InTech, 2012. P. 59– 88. DOI:10.5772/48292
9. Takisawa R., Maruyama T., Nakazaki T., Kataoka K., Saito H., Koeda S., Nunome T., Fukuoka H., Kitajima A. Parthenocarpy in the tomato (Solanum lycopersicum L.) cultivar ‘MPK-1’ is controlled by a novel parthenocarpic gene // Horticult. J. 2017. V. 86. № 4. P 487–492. DOI: 10.2503/hortj.OKD-042
10. Ueta R., Abe C., Watanabe T., Sugano S.S., Ishihara R., Ezura H., Osakabe Y., Osakabe K. Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9 // Sci. Rep. 2017. V. 7. P. 507. DOI: 10.1038/s41598-017-00501-4