eISSN: 2221-6197 DOI: 10.31301/2221-6197

METAGENOMIC ANALYSIS OF RNA VIRUS SEQUENCES - PANACEA FOR RAPID SCREENING OF EFFECTIVE PRIMERS FOR VIRAL DIAGNOSIS

Year: 2017

Pages: 384-388

Number: Volume 9, issue 4

Type: scientific article

Topic: Articles

Authors: Kalashnikov A.E. !, Gladyr E.A.!

Summary:

Here we describe a method for genomics that includes data filtering and final preparation of the viral consensus sequences. The outputs could be therefore used for the calculation of primers for viral detection. This method allows a simultaneous analysis of multiple sequences to obtain the variability at each site at whole-genome level or at the most complete viral consensus. We show two examples for diagnosing RNA viruses infecting bees and cattle, which we have successfully tested on the biological samples collected in Russia.

Keywords:

retroviruses, metagenomics, virus variability, Apis mellifera, Bos taurus, leukemia virus, mutation kinetics

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eISSN: 2221-6197 DOI: 10.31301/2221-6197