Biomics

In this review we consider the components of bacterial cells involved in the early stages of the formation of plant-microbial interactions. For the successful attachment of bacterial cells, the synthesis of bacterial surface polysaccharides (exopolysaccharides (EPS), lipopolysaccharides (LPS), capsular polysaccharides (KPS, K-antigens)), plant lectins and bacterial adhesins, including the RapA1 protein, is necessary. All these components are molecules mediators in plant-microbial interactions and are critical for the formation of effective symbiosis.

Our goal was to investigate the possibility of using the bacterial adhesin RapA1 R. leguminosarum as a tool for creating artificial associations of cultivated plants with PGPR microorganisms.

To achieve this goal, recombinant R. leguminosarum rhizobium strains with increased production of RapA1 protein, as well as R. galegae and E. coli strains, in which the protein was synthesized de novo, were obtained. Studies have shown an increase in the ability of modified strains to autoagglutination, which indicates an increase in adhesive properties.

The bacterial adhesin RapA1 of R. leguminosarum can be used as a tool for improving the efficiency of the formation of existing endosymbiosis, as well as for creating associative symbiotic systems de novo. Moreover, the spectrum of strains is not limited to rhizobia, since this adhesive does not have strict specificity with respect to these microsymbionts. In addition to the properties of the adhesive, the RapA1 protein also has an agglutinating capacity, which is necessary in the processes of biofilm formation on the surface of plant roots, which also increases the competitiveness of the introduced rhizobium strains in agrocenosis.

PGPR microorganisms, rhizobia, RapA1 adhesin, bacterial autoagglutination, biofilm, EPS, LPS, KPS

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