Biomics

The effect of the induction medium composition on the efficiency of callusogenesis and the subsequent ability of the resulting calli for regeneration in barley cv. Steptoe and its ABA-deficient mutant AZ34 was studied. A strong influence of a number of substances on the growth rates of calli and their ability to regeneration was noted. It has been established that for barley cv. Steptoe the addition of 0.2 mg/l of 24- epibrassinolide as a growth regulator stimulating synthesis of endogenous cytokinins in the Murashige-Skoog base medium and an increase in the copper sulfate content to 12.5 mg/l were more effective. For AZ34 additional supplementation of 0.5 mg/l ABA in induction medium is required. The possibility of using the hormonal mutant calli as models for studying the hormones interaction of in plant development is discussed.

barley, callus, regeneration ability, ABA, 24-epibrassinolid, copper sulphate

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