Year: 2017
Pages: 203-228
Number: Volume 9, issue 3
Type: scientific article
Topic: Articles
Authors: Chemeris D.A., Kiryanova O.Yu., Gerashchenkov G.A., Kuluev Bulat R., Rozhnova N.A., Matniyazov R.T, Baymiev Al.Kh, Baymiev An.Kh., Gubaidullin I.M., Chemeris A.V.
The features of numerous programs for gRNA designing with their actual web pages and the stages of work with them are described. For a number of programs are described more detailed, that is reflected in a special Appendix. Programs for mutations search after CRISPR/Cas editing of genomes of various organisms are listed. Databases containing previously selected gRNA for a number of genomes of plants and animals, including humans are considered. The analysis of almost all the existing literature on computer designing of gRNA and relevant Internet resources are shown.
CRISPR, Cas, PAM, gRNA, spacer, protospacer, On-target site, Off-target site, database, software, web-resource