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Биоинформационные ресурсы для CRISPR/Cas редактирования геномов

Год: 2017

Страницы: 203-228

Номер: Том 9, №3

Тип: научная статья

Рубрика: Статьи

Авторы: Баймиев Андрей Ханифович, Кирьянова (Забейворота) Ольга Юрьевна, Матниязов Рустам Тахирович, Валеев Альберт Шакирьянович, Баймиев Алексей Ханифович, Губайдуллин Ирек Марсович, Чемерис Дмитрий Алексеевич, Чемерис Алексей Викторович

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Аннотация:

Дана краткая характеристика CRISPR-локусов, встречающихся приблизительно у половины бактерий и у большинства архей. Показана их типичная организация, важным элементом которой служат CRISPR-кассеты, содержащие уникальные спейсеры, перемежающиеся одинаковыми прямыми повторами. Кратко рассмотрены специализированные программы поиска CRISPR-кассет в секвенированных геномах микроорганизмов и в метагеномных данных путем выявления в них повторяющихся участков. Приведены актуальные web-страницы таких программ и в табличной форме указаны их предназначения и возможности. Отмечены базы данных по CRISPR-локусам с указанием их web-адресов. Проведен анализ практически всей имеющейся литературы по данному вопросу и соответствующие интернет-ресурсы.

Ключевые слова:

CRISPR/Cas система, CRISPR-локус, CRISPR-кассеты, спейсер, квазитандемные повторы, компьютерная программа, web-ресурс, база данных

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eISSN: 2221-6197 DOI: 10.31301/2221-6197