Metagenomic algorithm for creating the diagnostic kits for the detection of RNA viruses of honey bees
Year: 2016
Pages: 36-39
Number: Volume 8, issue 1
Type: scientific article
Topic: Articles
Authors: Kalashnikov A.E. !, Burmistrova L.A.!, Borodachev V.I.!, Maslennikova V.I.!, Gladyr E.A.!, Korolev A.V.!
Summary:
Application of bio-information technology and analysis techniques is necessary for creation of a new generation of diagnostics. Establishment of such tests requires a new approach to selective parts calculations and selection of reagents. Example, presented in this work, allows being more effective on the way in identifying of retroviruses and analysis of their genomes. The use of this diagnosticum in practice found a high degree of infection of tribal and private apiaries in the Ryazan region.
Keywords:
retroviruses, metagenomics, virus variability, Apis mellifera, mutation kinetics, diagnostics.
References:
- Зиновьева Н.А., Костюнина О.В., Гладырь Е.А., Банникова А.Д., Харзинова В.Р., Ларионова П.В., Шавырина К.М., Эрнст Л.К. Роль ДНК-маркеров признаков продуктивности сельскохозяйственных животных // Зоотехния. 2010. №1. C. 8-10.
- Калашников А.Е., Удина И.Г. Эпидемиологическое состояние некоторых пасек при инфицировании семей пчел РНК-содержащими вирусами // VetPharma. Pharm Animals, 2014(1). C. 80-84.
- Удина И.Г., Кунижева С.С., Гришечкин А.Е., Калашников А.Е., Учаева В.С., Кривцов Н.И., Злобин В.И Обнаружение вируса деформации крыла у медоносной пчелы Apis mellifera L. на пасеках в Московской области методом ОТ-ПЦР // Вопросы вирусологии, 2010(5). C. 37-40.
- Berenyi O., Bakonyi N., Derakhshifar I., Koglberger H., Nowotny N. Occurrence of six honeybee viruses in diseased Austrian apiaries // Appl Envir Microbiology. V.72. N.4. P. 2414-2420.
- Chen Y.P., HigginsJ. A., Feldlaufer M.F. Quantitative Real-Time Reverse Transcription-PCR Analysis of Deformed Wing Virus Infection in the Honeybee (Apis mellifera) // Appl. Environ. Microbiol. 2005. V.71. N.1. P. 436-441.
- DeMirandaJ.R., GenerschE. Deformedwingvirus // JIntertebrPatology. 2010. V.103. N.1. P. 48-61.
- Livak K.J., Schmittgen T.D., Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR data // Methods. V.25. P. 402-408.
- Mackay I.M., Real-time PCR in the microbiology laboratory // Clinical Microbiology and Infection. V.10. I.3. P.190-212.