Biomics

Various methods of PCR products cloning are described, including the quite exotic. Review is carried out for a large number of the methodical experimental works devoted to amplicon cloning in phage and plasmid vectors. All existing methods of the amplicon cloning can be subdivided into several groups, differs by blunt or sticky ends cloning, A/Т‑ligation and recombination. Each such approach is realized by multiple ways, differing by DNA ligase using, directed and non-directed inserts, etc. Advantages and shortcomings of the main methods are briefly considered.

PCR, amplicon, DNA polymerase, cloning, vector, Т-vector, DNA ligase, topoisomerase, recombination

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