Biomics

The analysis of the available methodological literature devoted to false-negative results in PCR is carried out. It focuses on the inhibition of PCR with substances of different nature, as well as improvement of the amplification process using certain additives and with the help of various DNA polymerases, including genetically engineered modified variants of them.

PCR, reverse transcriptase PCR, RT-PCR, false-negative PCR, amplicon, DNA polymerase, reverse transcriptase, DNA, RNA, nucleic acids, extraction, inhibitors, facilitators

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