Biomics

Проведен анализ имеющейся литературы методического плана, посвященной вопросам возникновения ложно-негативных результатов при проведении ПЦР. Главное внимание уделено ингибированию реакции веществами различной природы, а также улучшению процесса амплификации с помощью некоторых добавок и за счет применения различных, в том числе, генно-инженерным путем модифицированных ДНК-полимераз.

ПЦР, обратно-транскрипционная ПЦР, ОТ-ПЦР, ложно-негативная ПЦР, ампликон, ДНК-полимераза, обратная транскриптаза, ДНК, РНК, нуклеиновые кислоты, экстракция, ингибиторы, усилители

1. Бикбулатова С.М., Мингазетдинова С.Р., Чемерис А.В., Вахитов В.А. Эволюция in vitro – есть ли предел методам «перетасовки» генов? // Успехи современной биологии. 2009. Т.129. С. 323-335.

2. Бикбулатова С.М., Чемерис Д.А., Никоноров Ю.М., Машков О.И., Гарафутдинов Р.Р., Чемерис А.В., Вахитов В.А. Способы детекции результатов полимеразной цепной реакции в режиме реального времени // Вестник Башкирского университета, 2012, Т. 17. С. 59-67.

3. Мавзютов А.Р., Бондаренко В.М., Латкин А.Т. Ингибиторы полимеразной цепной реакции // Журн. микробиол. 2003. №3. С.93-98.

4. Магданов Э.Г., Гарафутдинов Р.Р., Чемерис А.В. Микрожидкостная ПЦР // Вестник Башкирского университета. 2013. Т. 18. в печати.

5. Творогова М.Г., Гущин А.Е. ПЦР исследования в лабораторной диагностике: вопросы обеспечения качества // Клин. лаб. диагн. 2006. №12. С.38-41.

6. Чемерис А.В., Магданов Э.Г., Гарафутдинов Р.Р., Вахитов В.А. Как исключить появление ложно-позитивных результатов при проведении полимеразной цепной реакции // Вестник биотехнологии и физико-химической биологии. 2012. Т.8. С.31-42.

7. Чемерис Д.А., Магданов Э.Г., Машков О.И., Гарафутдинов Р.Р., Чемерис А.В. ПЦР с отложенным (горячим или задержанным) стартом // Биомика. 2011. Т.2., С.1-8.

8. Abolmaaty A., Gu W., Witkowsky R., Levin R.E. The use of activated charcoal for the removal of PCR inhibitors from oyster samples // J. Microbiol. Methods. 2007. V. 68. P. 349-352.

9. Abu Al-Soud W., Râdström P. Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples // Appl. Environ. Microbiol. 1998. V. 64. P. 3748-3753.

10. Akane A., Matsubara K., Nakamura H., Takahashi S., Kimura K. Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification // J. Forensic Sci. 1994. V. 39. P. 362-372.

11. Akane A., Shiono H., Matsubara K., Nakamura H., Hasegawa M., Kagawa M. Purification of forensic specimens for the polymerase chain reaction (PCR) analysis // J. Forensic Sci. 1993. V. 38. P. 691-701.

12. Alaeddini R. Forensic implications of PCR inhibition--A review // Forensic Sci. Int. Genet. 2012. V. 6. P. 297-305.

13. Al-Soud W.A., Jönsson L.J., Râdström P. Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR // J. Clin. Microbiol. 2000. V. 38. P. 345-350.

14. Al-Soud W.A., Rådström P. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat // J. Clin. Microbiol. 2000. V. 38. P. 4463-4470.

15. Al-Soud W.A., Rådström P. Purification and characterization of PCR-inhibitory components in blood cells // J. Clin. Microbiol. 2001. V. 39. P. 485-493.

16. Al-Soud WA, Ouis IS, Li DQ, Ljungh S, Wadström T. Characterization of the PCR inhibitory effect of bile to optimize real-time PCR detection of Helicobacter species // FEMS Immunol Medю Microbiol. 2005. V. 44. P. 177-182

17. Amicosante M., Richeldi L., Trenti G., Paone G., Campa M., Bisetti A., Saltini C. Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin // J. Clin. Microbiol. 1995. V. 33. P. 629-630.

18. Arnal C., Ferré-Aubineau V., Besse B., Mignotte B., Schwartzbrod L., Billaudel S. Comparison of seven RNA extraction methods on stool and shellfish samples prior to hepatitis A virus amplification // J. Virol. Methods. 1999. V. 77. P. 17-26.

19. Baar C., d'Abbadie M., Vaisman A., Arana M.E., Hofreiter M., Woodgate R., Kunkel T.A., Holliger P. Molecular breeding of polymerases for resistance to environmental inhibitors // Nucleic Acids Res. 2011. V. 39. e51.

20. Bachmann L., Scholz M., Broghammer M., Giddings I., Pusch C.M. Voltage-induced release of nucleic acids from palaeontological samples // Electrophoresis. 2000. V. 21. P. 1488-1492.

21. Ballagi-Pordány A., Belák S. The use of mimics as internal standards to avoid false negatives in diagnostic PCR // Mol. Cell Probes. 1996. V. 10. P. 159-164.

22. Baskaran N., Kandpal R.P, Bhargava A.K, Glynn M.W., Bale A., Weissman S.M. Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content // Genome Res.1996. V. 6. P. 633-638.

23. Bélec L., Authier J., Eliezer-Vanerot M.C., Piédouillet C., Mohamed A.S., Gherardi R.K. Myoglobin as a polymerase chain reaction (PCR) inhibitor: a limitation for PCR from skeletal muscle tissue avoided by the use of Thermus thermophilus polymerase // Muscle Nerve. 1998. V. 21. P. 1064-1067.

24. Bickley J., Short J.K., McDowell D.G., Parkes H.C. Polymerase chain reaction (PCR) detection of Listeria monocytogenes in diluted milk and reversal of PCR inhibition caused by calcium ions // Lett. Appl. Microbiol. 1996. V. 22. P. 153-158.

25. Boesenberg-Smith K.A., Pessarakli M.M., Wolk D.M. Assessment of DNA Yield and Purity: an overlooked detail of PCR troubleshooting // Clin. Microbiol. Newsletter. 2012. V. 34. P. 1-6.

26. Bonot S., Courtois S., Block J.C., Merlin C. Improving the recovery of qPCR-grade DNA from sludge and sediment // Appl. Microbiol. Biotechnol. 2010. V. 87. P. 2303-2311.

27. Bourke M.T., Scherczinger C.A., Ladd C., Lee H.C. NaOH treatment to neutralize inhibitors of Taq polymerase // J. Forensic. Sci. 1999. V. 44. P. 1046-1050.

28. Braid M.D., Daniels L.M., Kitts C.L. Removal of PCR inhibitors from soil DNA by chemical flocculation // J. Microbiol. Methods. 2003. V. 52. P. 389-393.

29. Buffone G.J., Demmler G.J., Schimbor C.M., Greer J. Improved amplification of cytomegalovirus DNA from urine after purification of DNA with glass beads // Clin. Chem. 1991. V. 37. P. 1945-1949.

30. Castella V., Kummer D., Gehrig C., Hall D. DNA extraction using the QIAsymphony: Evaluation of PCR inhibitor removal // Forensic Sci. Int. Genet. 2011. V. 3. e69–e70.

31. Chakrabarti R., Schutt C.E. Novel sulfoxides facilitate GC-rich template amplification // Biotechniques. 2002. P. 32. P. 866, 868, 870-872, 874.

32. Chakrabarti R., Schutt C.E. The enhancement of PCR amplification by low molecular-weight sulfones // Gene. 2001. V. 274. P. 293-298.

33. Chakrabarti R., Schutt C.E. The enhancement of PCR amplification by low molecular weight amides // Nucleic Acids Res. 2001. V. 29. P. 2377-2381.
34. Chernesky M.A., Jang D., Sellors J., Luinstra K., Chong S., Castriciano S., Mahony J.B. Urinary inhibitors of polymerase chain reaction and ligase chain reaction and testing of multiple specimens may contribute to lower assay sensitivities for diagnosing Chlamydia trachomatis infected women // Mol. Cell Probes. 1997. V. 11. 243-249.
35. Chevet E., Lemaître G., Katinka M.D. Low concentrations of tetramethylammonium chloride increase yield and specificity of PCR // Nucleic Acids Res. 1995. V. 23. P. 3343-3344.
36. Cone R.W., Hobson A.C., Huang M.L. Coamplified positive control detects inhibition of polymerase chain reactions // J. Clin. Microbiol. 1992. V. 30. P. 3185-3189.
37. Costello M.T., Schumm J.W. A single assay for human-specific guantification of less than 1 pg DNA and detection of the presence of PCR inhibitors in forensic samles // Int. Congress Series. 2006. V. 1288. P. 753-755.
38. de Castillo Agudo L., Gavidia I., Pérez-Bermúdez P., Segura J. PEG precipitation, a required step for PCR amplification of DNA from wild plants of Digitalis obscura L. // Biotechniques. 1995. V. 18. P. 766-768.
39. de Lomas J.G., Sunzeri F.J., Busch M.P. Falsenegative results by polymerase chain reaction due to contamination by glove powder // Transfusion. 1992. V. 32. P. 83-85.
40. Demeke T., Adams R.P. The effects of plant polysaccharides and buffer additives on PCR // Biotechniques. 1992. V. 12. P. 332-334.
41. Demeke T., Jenkins G.R. Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits // Anal Bioanal Chem. 2010. V. 396. P. 1977-1990.
42. Deneer H.G., Knight I. Inhibition of the polymerase chain reaction by mucolytic agents // Clin. Chem. 1994. V. 40. P. 171-172.
43. Eckhart L., Bach J., Ban J., Tschachler E. Melanin binds reversibly to thermostable DNA polymerase and inhibits its activity // Biochem. Biophys. Res. Commun. 2000. V. 271. P. 726-730.
44. Eilert K.D., Foran D.R. Polymerase resistance to polymerase chain reaction inhibitors in bone* // J. Forensic Sci. 2009. V. 54. P. 1001-1007.
45. Ellison S.L., Emslie K.R., Kassir Z. A standard additions method reduces inhibitor-induced bias in quantitative real-time PCR // Anal. Bioanal. Chem. 2011. V. 401. P. 3221-3227.
46. Faber K.L., Person E.C., Hudlow W.R. PCR inhibitor removal using the NucleoSpin(®) DNA Clean-Up XS kit // Forensic Sci. Int. Genet. 2013. V. 7. P. 209-213.
47. Fan X.Y., Lü G.Z., Wu L.N., Chen J.H., Xu W.Q., Zhao C.N., Guo S.Q. A modified single-tube onestep product-enhanced reverse transcriptase (mSTOS-PERT) assay with heparin as DNA polymerase inhibitor for specific detection of RTase activity // J. Clin. Virol. 2006. V. 37. P. 305-312.
48. Fang G., Hammar S., Grumet R. A quick and inexpensive method for removing polysaccharides from plant genomic DNA // Biotechniques. 1992. V. 13. P. 52-54, 56.
49. Fehlmann C., Krapf R., Solioz M. Reverse transcriptase can block polymerase chain reaction // Clin. Chem. 1993. V. 39. P. 368-369.
50. Foley C., O'Farrelly C., Meade K.G. Technical note: Comparative analyses of the quality and yield of genomic DNA from invasive and noninvasive, automated and manual extraction methods // J. Dairy Sci. 2011. V.94. P.3159-3165.
51. Fredericks D.N., Relman D.A. Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate // J. Clin. Microbiol. 1998. V. 30. P. 2810-2816.
52. Goodyear P.D., MacLaughlin-Black S., Mason I.J. A reliable method for the removal of co-purifying PCR inhibitors from ancient DNA // Biotechniques. 1994. V. 16. P. 232-235. 5
53. Hall L.M., Slee E., Jones D.S. Overcoming polymerase chain reaction inhibition in old animal tissue samples using ethidium bromide // Anal. Biochem. 1995. V. 225. P. 169-172.
54. Hänni C., Brousseau T., Laudet V., Stehelin D. Isopropanol precipitation removes PCR inhibitors from ancient bone extracts // Nucleic Acids Res. 1995. V. 23. P. 881-882.
55. Hartman L.J., Coyne S.R., Norwood D.A. Development of a novel internal positive control for Taqman based assays // Mol. Cell. Probes. 2005. V. 19. P. 51-59.
56. Hedman J., Dufva C., Noren L., Ansell C., Albinsson L., Ansell R. Applying a PCR inhibitor tolerant DNA polemerase blend in forensic DNA profiling // Forensic Sci. Int. Genet. 2011. V. 3. e349-e350.
57. Hedman J., Nordgaard A., Dufva C., Rasmusson B., Ansell R., Rådström P. Synergy between DNA polymerases increases polymerase chain reaction inhibitor tolerance in forensic DNA analysis // Anal. Biochem. 2010. V. 405. P. 192-200.
58. Hedman J., Nordgaard A., Rasmusson B., Ansell R., Rådström P. Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modeling of DNA profiles // Biotechniques. 2009. V. 47. P. 951-958.
59. Hengen P.N. Optimizing multiplex and LA-PCR with betaine // Trends Biochem. Sci. 1997. V. 22. P. 225-226.
60. Henke W., Herdel K., Jung K., Schnorr D., Loening S.A. Betaine improves the PCR amplification of GC-rich DNA sequences // Nucleic Acids Res. 1997. V. 25. P. 3957-3958.
61. Herrera A., Cockell C.S. Exploring microbial diversity in volcanic environments: a review of methods in DNA extraction // J. Microbiol. Methods. 2007. V.70. P.1-12.
62. Huggett J.F., Novak T., Garson J.A., Green C., Morris-Jones S.D., Miller R.F., Zumla A.. Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon // BMC Res Notes. 2008. V. 1. P. 70.
63. Hung T., Mak K., Fong K. A specificity enhancer for polymerase chain reaction // Nucleic Acids Res. 1990. V. 18. P. 4953.
64. Hyun C., Filippich L.J., Hughes I. The inhibitory effect of pentobarbitone on reverse transcriptionPCR // J. Biochem. Biophys. Methods. 2005. V. 62. P. 63-68.
65. Kang J., Lee M.S., Gorenstein D.G. The enhancement of PCR amplification of a random sequence DNA library by DMSO and betaine: application to in vitro combinatorial selection of aptamers // J. Biochem. Biophys. Methods. 2005. V. 64. P. 147-151.
66. Katcher H.L., Schwartz I. A distinctive property of Tth DNA polymerase: enzymatic amplification in the presence of phenol // Biotechniques. 1994. V. 16. P. 84-92.
67. Kermekchiev M.B., Kirilova L.I., Vail E.E., Barnes W.M. Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples // Nucleic Acids Res. 2009. V. 37. e40.
68. Kern M., Böhm S., Deml L., Wolf H., Reischl U., Niller H.H. Inhibition of Legionella pneumophila PCR in respiratory samples: a quantitative approach // J. Microbiol. Methods. 2009. V. 79. P. 189-193.
69. Kim S., Labbe R.G., Ryu S. Inhibitory effects of collagen on the PCR for detection of Clostridium perfringens // Appl. Environ. Microbiol. 2000. V. 66. P. 1213-1215.
70. Kodzius R., Xiao K., Wu J., Yi X., Gong X., Foulds I.G., Wen W. Inhibitory effect of common microfluidic materials on PCR outcome // Sensors and Actuators B: Chemical. 2012. V. 161. P. 349- 358.
71. Kontanis E.J., Reed F.A. Evaluation of real-time PCR amplification efficiencies to detect PCR inhibitors // J. Forensic Sci. 2006. V. 51. P. 795- 804.
72. Koonjul P.K., Brandt W.F., Farrant J.M., Lindsey G.G. Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA // Nucleic Acids Res. 1999. V. 27. P. 915-916.
73. Kosch T.A., Summers K. Techniques for minimizing the effects of PCR inhibitors in the chytridiomycosis assay // Mol. Ecol. Resour. 2012 Dec 14. (Epub ahead of print).
74. Kovárová M., Dráber P. New specificity and yield enhancer of polymerase chain reactions // Nucleic Acids Res. 2000. V. 28. E70.
75. Kreader C.A. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein // Appl. Environ. Microbiol. 1996. V. 62. P. 1102-1106.
76. Lantz P-G., Matsson M., Wadstrom T., Rådström P. Removal of PCR inhibitors from human faecal samples through the use of an aqueous two-phase system for sample preparation prior to PCR // J. Microbiol. Methods. 1997. V. 28. P. 159-167.
77. Lau J.Y.N., Qian K-P., Wu P.C., Davis G.L. Ribonucleotide vanadyl complexes inhibit polymerase chain reaction // Nucleic Acids Res. 1999. V. 27. P. 915-916.
78. Liu Q., Thorland E.C., Sommer S.S. Inhibition of PCR amplification by a point mutation downstream of a primer // Biotechniques. 1997. V. 22. P. 292-4, 296, 298, passim.
79. Maurer JJ. Rapid detection and limitations of molecular techniques // Annu. Rev. Food Sci. Technol. 2011. V. 2. P. 259-279.
80. McCormack J.M., Sherman M.L., Maurer D.H. Quality control for DNA contamination in laboratories using PCR-based class II HLA typing methods // Hum. Immunol. 1997. V. 54. P. 82-88.
81. McOrist A.L., Jackson M., Bird A.R. A comparison of five methods for extraction of bacterial DNA from human faecal samples // J. Microbiol. Methods. 2002. V. 50. P. 131-139.
82. Michaels S.D., John M.C., Amasino R.M. Removal of polysaccharides from plant DNA by ethanol precipitation // Biotechniques. 1994. V. 17. P. 274-276.
83. Miller D.N. Evaluation of gel filtration resins for the removal of PCR-inhibitory substances from soils and sediments // J. Microbiol. Methods. 2001. V. 44. P. 49-58.
84. Monteiro L., Bonnemaison D., Vekris A., Petry K.G., Bonnet J., Vidal R., Cabrita J., Mégraud F. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model // J. Clin. Microbiol. 1997. V. 35. P. 995-998.
85. Monteiro L., Gras N., Megraud F. Magnetic immuno-PCR assay with inhibitor removal for direct detection of Helicobacter pylori in human feces // J. Clin. Microbiol. 2001. P. 39. P. 3778- 3780.
86. Monteiro L., Gras N., Vidal R., Cabrita J., Mégraud F. Detection of Helicobacter pylori DNA in human feces by PCR: DNA stability and removal of inhibitors // J. Microbiol. Methods. 2001. V. 45. P. 89-94.
87. Moreira D. Efficient removal of PCR inhibitors using agarose-embedded DNA preparations // Nucleic Acids Res. 1998. V. 26. P. 3309-3310.
88. Musso M., Bocciardi R., Parodi S., Ravazzolo R., Ceccherini I. Betaine, dimethyl sulfoxide, and 7- deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences // J. Mol. Diagn. 2006. V. 8. P. 544-550.
89. Nolan T., Hands R.E., Ogunkolade W., Bustin S.A. SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations // Anal. Biochem. 2006. V. 351. P. 308-310.
90. Oikarinen S., Tauriainen S., Viskari H., Simell O., Knip M., Virtanen S., Hyöty H. PCR inhibition in stool samples in relation to age of infants // J. Clin. Virol. 2009. V. 44. P. 211-214.
91. Opel K.L., Chung D., McCord B.R. A study of PCR inhibition mechanisms using real time PCR // J. Forensic Sci. 2010. V. 55. P. 25-33.
92. Pallen M.J., Puckey L.H., Wren B.W. A rapid, simple method for detecting PCR failure // PCR Methods and Application. 1992. V. 2. P. 91-92.
93. Panaccio M., Lew A. PCR based diagnosis in the presence of 8% (v/v) blood // Nucleic Acids Res. 1991. V. 19. P. 1151.
94. Pao C.C., Hor J.J., Tsai P.L., Horng M.Y. Inhibition of in vitro enzymatic DNA amplification reaction by ultra-violet light irradiation // Mol. Cell. Probes. 1993. V. 7. P. 217-219.
95. Phillips K., McCallum N., Welch L. A comparison of methods for forensic DNA extraction: Chelex-100 and the QIAGEN DNA Investigator Kit (manual and automated) // Forensic Sci. Int. Genet. 2012. V. 6. P. 282-285.
96. Pikaart M.J., Villeponteau B. Suppression of PCR amplification by high levels of RNA // Biotechniques. 1993. V. 14. P. 24-25.
97. Poddar S.K., Sawyer M.H., Connor J.D. Effect of inhibitors in clinical specimens on Taq and Tth DNA polymerase-based PCR amplification of influenza A virus // J. Med. Microbiol. 1998. V. 47. P. 1131-1135.
98. Poli F., Cattaneo R., Crespiatico L., Nocco A., Sirchia G. A rapid and simple method for reversing the inhibitory effect of heparin on PCR for HLA class II typing // PCR Methods Appl. 1993. V. 2. P. 356-358.
99. Pomp D., Medrano J.F. Organic solvents as facilitators of polymerase chain reaction // Biotechniques. 1991. V. 10. P. 58-59.

100.Pontirolli A., Travis E.R., Sweeney F.P., Porter D., Gaze W.H., Mason S., Hibberd V., Holden J., Courtenay O., Wellington E.M.H. Pathogen quantitation in complex matrices: a multioperator comparison of DNA extraction methods with a novel assessment of PCR inhibition // PloS ONE. 2011. V.6. E17916 (p.1-11)/

101.Price K., Linge C. The presence of melanin in genomic DNA isolated from pigmented cell lines interferes with successful polymerase chain reaction: a solution // Melanoma Res. 1999. V. 9. P. 5-9.

102.Rådström P., Knutsson R., Wolffs P., Lövenklev M., Löfström C. Pre-PCR processing: strategies to generate PCR-compatible samples // Mol. Biotechnol. 2004. V. 26. P.133-146.

103.Ralser M., Querfurth R., Warnatz H.J., Lehrach H., Yaspo M.L., Krobitsch S. An efficient and economic enhancer mix for PCR // Biochem. Biophys. Res. Commun. 2006. V. 347. P. 747- 751.

104.Rapp D. DNA extraction from bovine faeces: current status and future trends // J. Appl. Microbiol. 2010. V.108. P.1485-1493.

105.Reysenbach A.L., Giver L.J., Wickham G.S., Pace N.R. Differential amplification of rRNA genes by polymerase chain reaction // Appl. Environ. Microbiol. 1992. V. 58. P. 3417-3418.

106.Rock C., Alum A., Abbaszadegan M. PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy // Appl. Environ. Microbiol. 2010. V. 76. P. 8102-8109.

107.Rohland N., Hofreiter M. Comparison and optimization of ancient DNA extraction // Biotechniques. 2007. V. 42. P. 343-352.

108.Rossen L., Nørskov P., Holmstrøm K., Rasmussen O.F. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNAextraction solutions // Int. J. Food Microbiol. 1992. V. 17. P. 37-45.

109.Ruano G., Pagliaro E.M., Schwartz T.R., Lamy K., Messina D., Gaensslen R.E., Lee H.C. Heatsoaked PCR: an efficient method for DNA amplification with applications to forensic analysis // Biotechniques. 1992. V. 13. P. 266- 274.

110.Sarkar G., Kapelner S., Sommer S.S. Formamide can dramatically improve the specificity of PCR // Nucleic Acids Res. 1990. V. 18. P. 7465.

111.Schneider S., Enkerli J., Widmer F. A generally applicable assay for the quantification of inhibitory effects on PCR // J. Microbiol. Methods. 2009. V. 78. P. 351-353.

112.Schnoor M., Voss P., Cullen P., Böking T, Galla H.J., Galinski E.A., Lorkowski S. Characterization of the synthetic compatible solute homoectoine as a potent PCR enhancer // Biochem. Biophys. Res. Commun. 2004. V. 322. P. 867-872.

113.Scholz M., Giddings I., Pusch C.M. A polymerase chain reaction inhibitor of ancient hard and soft tissue DNA extracts is determined as human collagen type I // Anal. Biochem. 1998. V. 259. P. 283-286.

114.Schrader C., Schielke A., Ellerbroek L., Johne R. PCR inhibitors - occurrence, properties and removal // J. Appl. Microbiol. 2012. V. 113. P. 1014-1026.

115.Schriewer A., Wehlmann A., Wuertz S. Improving qPCR efficiency in environmental samples by selective removal of humic acids with DAX-8 // J. Microbiol. Methods. 2011. V. 85. P. 16-21.

116.Sidhu M.K., Liao M.J., Rashidbaigi A. Dimethyl sulfoxide improves RNA amplification // Biotechniques. 1996. V. 21. P. 44-47.

117.Simon M.C., Gray D.I., Cook N. DNA Extraction and PCR Methods for the Detection of Listeria monocytogenes in Cold-Smoked Salmon // Appl. Environ. Microbiol. 1996. V. 62. P. 822-824.

118.Sørensen E., Hansen S.H., Eriksen B., Morling N. Application of thiopropyl sepharose 6B for removal of PCR inhibitors from DNA extracts of a thigh bone recovered from the sea // Int. J. Legal. Med. 2003. V. 117. P. 245-247.

119.Stangegaard M., Hjort B.B., Hansen T.N., Hansen A.J., Morling N. Automated extraction of DNA from clothing // Forensic Sci. Int. Genet. 2011. V. 3. e403-e404.

120.Straub T.M., Pepper I.L., Gerba C.P. Removal of PCR inhibiting substances in sewage amended soil // Water science and Technology. 1995. V. 31. P. 311-315.

121.Sturr M.G., Marquis R.E. Comparative acid tolerances and inhibitor sensitivities of isolated FATPases of oral lactic acid bacteria // Appl. Environ. Microbiol. 1992 V. 58. P. 2287-2291.

122.Suenaga E., Nakamura H. Evaluation of three methods for effective extraction of DNA from human hair // J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2005. V. 820. P. 137-141.

123.Sur K., McFall S.M., Yeh E.T., Jangam S.R., Hayden M.A., Stroupe S.D., Kelso D.M. Immiscible phase nucleic acid purification eliminates PCR inhibitors with a single pass of paramagnetic particles through a hydrophobic liquid // J. Mol. Diagn. 2010. V. 12. P. 620-628.

124.Suslov O., Steindler D.A. PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency // Nucleic Acids Res. 2005. V. 33. e181.

125.Sutlović D., Definis Gojanović M., Andelinović S., Gugić D., Primorac D. Taq polymerase reverses inhibition of quantitative real time polymerase chain reaction by humic acid // Croat. Med. J. 2005. V. 46. P. 556-562.

126.Tan S.C., Yiap B.C. DNA, RNA, and protein extraction: the past and the present // J. Biomed Biotechnol. 2009. V. 2009. ID 574398. 10p.

127.Thornton C.G., Passen S. Inhibition of PCR amplification by phytic acid, and treatment of bovine fecal specimens with phytase to reduce inhibition // J. Microbiol. Methods. 2004. P. 59. P. 43-52.

128.Tichopad A., Polster J., Pecen L., Pfaffl M.W. Model of inhibition of Thermus aquaticus polymerase and Moloney murine leukemia virus reverse transcriptase by tea polyphenols (+)- catechin and (-)-epigallocatechin-3-gallate // J. Ethnopharmacol. 2005. V. 99. P. 221-227.

129.van Doorn R., Klerks M.M., van Gent-Pelzer M.P., Speksnijder A.G., Kowalchuk G.A., Schoen C.D. Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel internal amplification control approach using Biotrove OpenArrays // Appl. Environ. Microbiol. 2009. V. 75. P. 7253-7260.

130.Varadaraj K., Skinner D.M. Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases // Gene. 1994. V. 140. P. 1-5.

131.Vázquez R., Steinberg M.L. Enhancement of PCRs by partial restriction digestion of genomic templates // Biotechniques. 1999. V. 26. P. 91-95.

132.Verheyen J., Kaiser R., Bozic M., Timmen-Wego M., Maier B.K., Kessler H.H. Extraction of viral nucleic acids: comparison of five automated nucleic acid extraction platforms // J. Clin. Virol. 2012. V. 54. P. 255-259.

133.Viltrop T., Krjutskov K., Palta P., Metspalu A. Comparison of DNA extraction methods for multiplex polymerase chain reaction // Anal. Biochem. 2010. V. 398. P. 260-262.

134.Wang X., Teng D., Tian F., Guan Q., Wang J. Comparison of three DNA extraction methods for feed products and four amplification methods for the 5'-junction fragment of Roundup Ready soybean // J. Agric. Food Chem. 2012. V. 60. P. 4586-4595.

135.Wang W., Wang H.B., Li Z.X., Guo Z.Y. Silicon inhibition effects on the polymerase chain reaction: a real-time detection approach // J. Biomed. Mater. Res. A. 2006. V.77. P.28-34.

136.Weissensteiner T., Lanchbury J.S. Strategy for controlling preferential amplification and avoiding false negatives in PCR typing // Biotechniques. 1996. V. 21. P. 1102-1108.

137.Wiedbrauk D.L., Werner J.C., Drevon A.M. Inhibition of PCR by aqueous and vitreous fluids // J. Clin. Microbiol. 1995. V. 33. P. 2643-2646.

138.Wilson I.G. Inhibition and facilitation of nucleic acid amplification // Appl. Environ. Microbiol. 1997. V. 63. P. 3741-3751.

139.Yedidag E.N., Koffron A.J., Mueller K.H., Kaplan B., Kaufman D.B., Fryer J.P., Stuart F.P., Abecassis M. Acyclovir triphosphate inhibits the diagnostic polymerase chain reaction for cytomegalovirus // Transplantation. 1996. V. 62. P. 238-242.

140.Yoon C-S., Glawe D.A. Pretreatment with Rnase to improve PCR amplification of DNA using 10- mer primers // Biotechniques. 1993. V. 14. P. 908- 910.

141.Zhang Z., Kermekchiev M.B., Barnes W.M. Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq // J. Mol. Diagn. 2010. V. 12. P. 152-161.

142.Zhu H., Qu F., Zhu L.H. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride // Nucleic Acids Res. 1993. V. 21. P. 5279-5280.