Biomics

Ability of 23 Bacillus subtilis endophytic strains to mobilize insoluble phosphates has been investigated. All strains dissolved calcium and iron phosphates, and 12 strains dissolve also aluminum phosphate. At the same time it has been revealed, that depending on a way experiment statement of it is possible to receive different results. It is shown, death of bacillus cells occurs during the cultivation in the liquid medium with the iron phosphate. That, apparently, is the reason for the bacterial strains inability to dissolve FePO₄ in a liquid medium  in contrast to the solid medium. One of the key mechanisms of dissolution of phosphates under the influence of endophytes is the production of organic di- and tricarboxylic acids to the culture medium. The presence and the quantitative ratio of these acids differed both depending on a strain, and on  consistence of the culture medium.

Bacillus subtilis endophytic strains, dissolution of phosphates, organic acids

1. Волошин С.А, Капрельянц А.С. Межклеточные взаимодействия в бактериальных популяциях // Биохимия. – 2004. – Т.69. – С. 1555–1564.
2. Недорезков В.Д. Биологическое обоснование применения эндофитных бактерий в защите пшеницы от болезней на Южном Урале: Дис. … д-ра с.-х. наук / ФГОУ ВПО «Башкирский государственный аграрный университет». – Уфа, 2003. – 280 с.
3. Сэги Й. Методы почвенной микробиологии. – М.: Колос, 1983. – 162 с.
4. Цавкелова Е.А., Климова С.Ю., Чердынцева Т.А., Нетрусов А.И. Микроорганизмы – продуценты стимуляторов роста растений и их практическое применение (обзор) // Прикладная биохимия и микробиология. – 2006. – Т.42. – С. 133–143.
5. Afif E., Matar A., Torrent J. Availability of phosphate applied to calcareous soils of West Asia and North Africa // Soil Sci. Soc. Am. J. – 1993. – V.57. – P. 756–760.
6. Antoun H., Prévost D. Ecology of plant growth promoting rhizobacteria // PGPR: Biocontrol and Biofertilization / Siddiqui Z.A. (ed.). – Springer, 2005. – P. 1-38.
7. Baca B.E., Elmerich C. Microbial production of plant hormones // Associative and Endophytic Nitrogen-fixing Bacteria and Cyanobacterial Associations / Elmerich C., Newton W.E. – Springer, 2007. – P. 113-143.
8. Bally R., Elmerich C. Biocontrol of plant diseases by associative and endophytic nitrogen-fixing bacteria // Associative and endophytic nitrogen-fixing bacteria and cyanobacterial assotiations / Elmerich, C., Newton, W.E. – Springer, 2007. – P. 171–190.
9. Bashan Y., Holguin G. Proposal for the division of plant growth-promoting rhizobacteria into two classifications: biocontrol-PGPB (plant growth-promoting bacteria) and PGPB // Soil Biol. Biochem. – 1998. – V.30. – P. 1225–1228.
10. Baud S., Bellec Y., Miquel M., Bellini C., Caboche M., Lepiniec L., Faure J.D., Rochat C. gurke and pasticcino3 mutants affected in embryo development are impaired in acetyl-CoA carboxylase // EMBO Rep. – 2004. – V.5. – P. 515–520.
11. Beauchamp C. J. Mode of action of plant growth-promoting rhizobacteria and their potential use as biological control agents // Phytoprotection. – 1993. – V.71. – P. 19–27.
12. Berg A.J.J., Radema M.H., Labadie R.P. Influence of acetate and malonate on the production of 1,8-dihydroxyanthracene derivatives in suspension cultures of Rhamnus purshiana // Planta. – 1988. – V.174. – P. 417–421.
13. Biggs A.R., El-Kholi M.M., El-Neshawy S., Nickerson R. Effects of calcium salts on growth, polygalacturonase activity, and infection of peach fruit by Monilinia fructicola // Plant Dis. – 1997. – V.81. – P. 399–403.
14. Biggs A.R. Effects of calcium salts on apple bitter rot caused by two Colletotrichum // Plant Dis. – 1999. – Vol. 83. – P. 1001–1005.
15. Blodgett A.B., Caldwell R.W., McManus P.S. Effects of calcium salts on the cranberry fruit rot disease complex // Plant Dis. – 2002. – V.86. – P. 747–752.
16. Chen H., Kim H.U., Weng H., Browse J. Malonil-CoA synthetase, encoded by ACYL ACTIVATING ENZYME13, is essential for growth and development of Arabidopsis // Plant Cell. – 2011. – V.23. – P. 2247–2262.
17. Compant S., Duffy B., Nowak J., Clément C., Barka E.A. Use plant growth-promoting bacteria for biocontrol of plant diseases principles, mechanism of action, and future prospects // Appl. Environ. Microbiol. – 2005. – V.71. – P. 4951–4959.
18. Davey M.E., O’Toole G.A. Microbial biofilms: from ecology to molecular genetics // Microbiol. Mol Biol. Rev. – 2000. – V.64. – P. 847–67.
19. Dobbelaere S., Okon Y. The plant growth-promoting effect and plant responses // Associative and Endophytic Nitrogen-fixing Bacteria and Cyanobacterial Associations / Elmerich C., Newton W.E. – Springer, 2007. – P. 145–170.
20. Fankem H., Nwaga D., Deubel A., Dieng L., Merbach W., Etoa F.X. Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon // African J. Biotechnol. – 2006. – V.56. – P. 2450–2460.
21. Hallman J., Berg G. Spectrum and population dynamic of bacterial root endophytes // Microbial root endophytes / Schulz B., Boyle C., Sieber T.N. - Springer-Verlag, 2006. – P. 15–31.
22. Hennequin C., Lalanne V., Daudon M., Lacour B., Drueke T. A new approach to studying inhibitors of calcium oxalate crystal growth // Urological Research. – 1993. – V.21. – P. 101–108.
23. Hervieux V., Yaganza E.S., Arul J., Tweddell R.J. Effect of organic and inorganic salts on the development of Helminthosporium solani, the causal agent of potato silver scurf // Plant Dis. – 2002. – V.86. – P. 1014–1018.
24. Hinsinger P. Bioavailability of soil inorganic P in the rhizosphere as affected by root-induced chemical changes: a review // Plant and Soil. – 2001. – V.237. – P. 173–195.
25. Huang L. F., Fang B. P., Luo Z. X., Chen J. Y., Zhang X. J., Wang Z. Y. First report of bacterial stem and root rot of sweetpotato caused by a Dickeya (Erwinia chrysanthemi) in China // Plant Dis. – 2010. – V.94. – P. 1503.
26. Jones D. L. Organic acids in the rhizosphere – a critical review // Plant Soil. – 1998. – V.205. – P. 25–44.
27. Kim Y.S. Malonate metabolism: biochemistry, molecular biology, physiology, and industrial application // J. Biochem. Mol. Biol. – 2002. – V.35. – P. 443–451.
28. Koike S. T., Henderson D. M., Azad H. R., Cooksey D. A., Little E. L. Bacterial blight of broccoli raab: A new disease caused by a pathovar of Pseudomonas syringae // Plant Dis. – 1998. – V.82. – P. 727–731.
29. Lambers H., Shane M.W., Cramer M.D., Pearse S.J., Veneklaas E.J. Root structure and functioning for efficient acquisition of phosphorus: matching morphological and physiological traits // Ann. Bot. – 2006. – V.98. – P. 693–713.
30. Lazarovits G., Nowak J. Rhizobacteria for improvement of plant growth and establishment // Hortiscience. – 1997. – V.32. – P. 188–192.
31. Li J., Copeland L. Role of malonate in chickpea // Phytochemistry. – 2000. – V.54. – P. 585–589.
32. Llama-Palacios A., López-Solanilla E., Rodríguez-Palenzuela P. Role of the PhoP-PhoQ system in the virulence of Erwinia chrysanthemi strain 3937: involvement in sensitivity to plant antimicrobial peptides, survival at acid pH, and regulation of pectolytic enzymes // J. Bacteriol. – 2005. – V.187. – P. 2157–2162.
33. Lombo F., Pfeifer B., Leaf T., Ou S., Kim Y.S., Cane D.E., Licari P., Khosla C. Enhancing the atom economy of polyketide biosynthetic processes through metabolic engineering // Biotechnol. Prog. – 2001. – V.17. – P. 612−617.
34. Mantelin S., Touraine B. Plant growth-promoting bacteria and nitrate availability: impact on root development and nitrate uptake // J. Exp. Bot. – 2004. – V.55. – P. 27–34.
35. Mehta S., Nautiyal C.S. An efficient method for quantitative screening of phosphate-solubilizing bacteria // Curr. Microbiol. – 2001. – V.43. – P. 51–56.
36. Mirik M., Aysan Y., Cetinkaya-Yildiz R., Sahin F., Saygili H. Watermelon as a new host of Pseudomonas viridiflava, causal agent of leaf and stem necrosis, discovered in Turkey // Plant Dis. – 2004. – V.88. – P. 907.
37. Newman L., Reynolds C.M. Bacteria and phytoremediation: new uses for endophytic bacteria in plants // Trends Biotechnol. – 2005. – V.23. – P. 6–8.
38. Olivier C., MacNeil C.R., Loria R. Application of organic and inorganic salts to field-grown potato tubers can suppress silver scurf during potato storage // Plant Dis. – 1999. – V.83. – P. 814–818.
39. Pang J., Cuin T., Shabala L., Zhou M., Mendham N., Shabala S. Effect of secondary metabolites associated with anaerobic soil conditions on ion fluxes and electrophysiology in barley roots // Plant Physiol. – 2007. – V.145. – P. 266–276.
40. Patten C.L., Glick B.R. Bacterial biosynthesis of indole-3-acetic acid // Can. J. Microbiol. – 1996. – V.42. – P. 207–220.
41. Peer W.A., Brown D.E., Tague B.W., Muday G.K., Taiz L., Murphy A.S. Flavonoid accumulation patterns of transparent testa mutants of Arabidopsis // Plant Physiol. – 2001. – V.126. – P. 536–548.
42. Pereg-Gerk L., Paquelin A., Gounon P., Kennedy I.R., Elmerich C. A transcriptional regulator of the LuxR-UhpA family, FlcA, controls flocculation and wheat root surface colonization by Azospirillum brasilense Sp7 // Mol. Plant-Microbe Interact. – 1998. – V. 11. – P. 177–187.
43. Plassard C., Dell B. Phosphorus nutrition of mycorrhizal trees // Tree Physiol. – 2010. – V.30. – P. 1129–1139.
44. Popova T.N., Pinheiro de Carvalho M.A. Citrate and isocitrate in plant metabolism // Biochim. Biophys. Acta. – 1998. – V.1364. – P. 307–325.
45. Rajendran L., Samiyappan R. Endophytic Bacillus species confer increased resistance in cotton against damping off disease caused by Rhizoctonia solani // Plant Pathol. J. – 2008. – V.7. – P. 1–12.
46. Rodriguez H., Fraga R., Gonzalez T., Bashan Y. Genetic of phosphate solubilization and its potencial applications for improving plant growth-promoting bacteria // Plant Soil. – 2006. – V.287. – P. 15–21.
47. Ryu C.-M., Farag M.A., Hu C.-H., Reddy M.S., Wei H.-X., Paré P.W., Kloepper J.W. Bacterial volatiles promote growth in Arabidopsis // Proc. Acad. Sci. USA. – 2003. – V.100. – P. 4927–4932.
48. Salomone J.-Y., Szegedi E., Cobanov P., Otten L. Tartrate utilization genes promote growth of Agrobacterium on grapevine // Mol. Plant-Microbe Interact. – 1998. – V.11. – P. 836–838.
49. Sexton A.C., Howlett B.J. Parallels in fungal pathogenesis on plant and animal hosts // Eukaryotic Cell. – 2006. – V.5. – P. 1941–1949.
50. Silk G.W., Matthews B.F. Soybean DapA mutations encoding lysine-insensitive dihydrodipicolinate synthase // Plant Mol. Biol. – 1997. – V.33. – P. 931–933.
51. Sturz A. V., Christie B. R., Matheson B. G., Nowak J. Biodiversity of endophytic bacteria which colonize red clover nodules, roots, stems and foliage and their influence on host growth // Biol. Fertil. Soils. – 1997. – V.25. – P. 13–19.
52. Taguchi G., Ubukata T., Nozue H., Kobayashi Y., Takahi M., Yamamoto H., Hayashida N. Malonylation is a key reaction in the metabolism of xenobiotic phenolic glucosides in Arabidopsis and tobacco // Plant J. – 2010. – V.63. – P. 1031–1041.
53. Whipps, J.M. Microbial interactions and biocontrol in the rhizosphere // J. Exp. Bot. – 2001. – V.52. – P. 487–511.
54. Yamakura F., Ikeda Y., Kimura K., Sasakawa T. Partial purification and some properties of pyruvate-aspartic semialdehyde condensing enzyme from sporulating Bacillus subtilis // J. Biochem. – 1974. – V.76. – P. 611–621.