Biomics

Исторически сложилось так, что сначала появилось выражение «ПЦР с горячим стартом» (hot start PCR), которое указывало на использование относительно высокой температуры на фактически предстартовом этапе ПЦР. Позднее появившееся понятие «ПЦР с задержанным стартом» (PCR time release), уже подчеркивало важность и других компонентов реакции, временное исключение которых приводило также к «задержке» старта ПЦР (момента начала непосредственного образования ампликонов) и повышало специфичность реакции. В статье в хронологическом порядке рассматриваются способы ПЦР с отложенным стартом, сдерживающих до определенного момента или работу ДНК полимеразы или отжиг праймеров.

ПЦР, «горячий» старт, отложенный старт, ДНК полимераза, праймер, дНТФ, ионы магния

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