eISSN: 2221-6197 DOI: 10.31301/2221-6197

Effect of ibuprofen on antioxidant system of wheat seedlings

Year: 2025

Pages: 238-244

Number: Volume 17, issue 3

Type: scientific article

Summary:

The effect of 100 μM ibuprofen (IBU) on the content of reactive oxygen species and activity of antioxidant enzymes in 3- and 5-day-old wheat plants under normal growing conditions was analyzed. The growth parameters of these plants were also analyzed. The preparation was found to stimulate plant growth and cause a balanced accumulation of superoxide anion (O2-) and hydrogen peroxide (H2O2). This is accompanied by activation of superoxide dismutase (SOD) and catalase (CAT), enzymes involved in neutralization of reactive oxygen species (ROS). For the first time, the obtained results indicate the ability of the drug to regulate the state of redox metabolism and provide grounds for considering IBU as a plant antioxidant.

Keywords:

Triticum aestivum L., growth indicators, ibuprofen, reactive oxygen species, plant growth regulator, antioxidant enzymes

References:

  1. Aebi H. Catalase in vitro. Methods in Enzymolgy. 1984. 105. 121–126. doi: 10.1016/S0076-6879(84)05016-3
  2. Baranova EN, Kononenko NV, Lapshin PV et al. Superoxide Dismutase Premodulates Oxidative Stress in Plastids for Protection of Tobacco Plants from Cold Damage Ultrastructure Damage. J. Mol. Sci. 2024. 25. 5544. doi: 10.3390/ijms25105544
  3. Beyer WF, Fridovich I. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions // Anal Biochem. 1987, V. 161. P. 559. doi: 10.1016/0003-2697(87)90489-1
  4. Bindschedler LV, Minibaeva F, Gardner SL et al. Early signalling events in the apoplastic oxidative burst in suspension cultured french bean cells involve cAMP and Ca2+. New Phytol. 151(1). 185-194. doi: 10.1046/j.1469-8137.2001.00170.x
  5. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976. 72. 248-254. doi: 10.1016/0003-2697(76)90527-3
  6. Dordio A, Ferro R, Teixeira D eet al. Study on the use of Typha for the phytotreatment of water contaminated with ibuprofen. International Journal of Environmental and Analytical Chemistry. 2011. 91(7-8). 654-667. doi: 10.1080/03067311003782708
  7. He Y, Langenhoff AAM, Sutton NB, Rijnaarts HHM et al. Metabolism of Ibuprofen by Phragmites australis: Uptake and Phytodegradation. Environ Sci Technol. 2017. 51(8). 4576-4584. doi: 10.1021/acs.est.7b00458.
  8. Iori V, Pietrini F, Zacchini M. Assessment of ibuprofen tolerance and removal capability in Populus nigra by in vitro culture. Journal of Hazardous Materials. 2012. 229–230. 217-223. doi: 10.1016/j.jhazmat.2012.05.097
  9. Kaznina NM, Repkina NS, Batova YV et al. Seeds treatment with salicylic acid increases gene expression and activity of antioxidant enzymes in wheat plants under zinc or copper deficiency. Doklady Rossijskoj akademii nauk. Nauki o žizni. 2024. 515(1). 81-86. doi: 10.31857/S2686738924020154 (In Russian)
  10. Madikizela LM, Botha TL, Kamika I et al. Uptake, Occurrence, and Effects of Nonsteroidal Anti-Inflammatory Drugs and Analgesics in Plants and Edible Crops. Agric. Food Chem. 2022. 70(1). 34–45. doi: 10.1021/acs.jafc.1c06499
  11. Maslennikova DR, Ivanov SP, Petrova SF. The Role of Antioxidant System Components in the Effect of Ibuprofen on Wheat Plants. Russian Journal of Plant Physiology. 2025. 72. 64. DOI: 10.1134/S1021443724610437
  12. Minibayeva FV, Gordon LK, Kolesnikov OP et al. Role of extracellular peroxidase in the superoxide production by wheat root cells. 2001. 217. 125. doi: 10.1007/BF01289421
  13. Niu L, Liao W. Hydrogen Peroxide Signaling in Plant Development and Abiotic Responses: Crosstalk with Nitric Oxide and Calcium. Front Plant Sci. 2016. 7. 230. doi: 10.3389/fpls.2016.00230
  14. Nurnaeimah N, Mat N, Suryati Mohd K et al. The Effects of Hydrogen Peroxide on Plant Growth, Mineral Accumulation, as Well as Biological and Chemical Properties of Ficus deltoidea. 2020. 10. 599. doi: 10.3390/agronomy10040599
  15. Opris O, Lung I, Soran ML et al. Investigating the effects of non-steroidal anti-inflammatory drugs (NSAIDs) on the composition and ultrastructure of green leafy vegetables with important nutritional values. Plant Physiol. Biochem. 2020. 151. 342–351. doi: 10.1016/j.plaphy.2020.03.046
  16. Rajput VD, Harish Singh RK, Verma KK et al. Recent Developments in Enzymatic Antioxidant Defence Mechanism in Plants with Special Reference to Abiotic Stress. Biology. 2021. 10(4). 267. doi: 10.3390/biology10040267
  17. Schmidt W, Redshaw CH. Evaluation of biological endpoints in crop plants after exposure to non‐steroidal anti‐inflammatory drugs (NSAIDs): Implications for phytotoxicological assessment of novel contaminants. Environ. Saf. 2015. 112. 212-222. doi: 10.1016/j.ecoenv.2014.11.008
  18. Schrader J, Shi P, Royer DL et al. Leaf size estimation based on leaf length, width and shape. Annals of Botany. 128(4). 395–406. doi: 10.1093/aob/mcab078
  19. Shan C, Liang Z. Jasmonic acid regulates ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. Plant Science. 2010. 178. 130-139. doi: 10.1016/j.plantsci.2009.11.002
  20. Sharma H, Shivani G, Anjali M. Paracetamol and ibuprofen effect on seed quality attributes of Triticum aestivum (wheat). J. Environ. Sci. 2018. 7(7). 44-48.
  21. Sharma I, Ahmad P. Catalase: A Versatile Antioxidant in Plants. Chapter 4 in book Oxidative Damage to Plants. 2014. 131-148. doi: 10.1016/B978-0-12-799963-0.00004-6
  22. Tan S, Di Donato M, Glanc M et al. Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development. Cell Rep. 2020. 33. 108463. doi: 10.1016/j.celrep.2020.108463
  23. Tarchevsky IA. Plant metabolism under stress. 2001. Feng Publishing House. 448 p. (In Russian)
  24. Wijaya L, Alyemeni M, Ahmad P et al. Ecotoxicological effects of ibuprofen on plant growth of Vigna unguiculata Plants. 2020. 9. 1473. doi: 10.3390/plants9111473
  25. Zhang T, Li N, Chen G et al. Stress symptoms and plant hormone-modulated defense response induced by the uptake of carbamazepine and ibuprofen in Malabar spinach (Basella alba). Sci. Total Environ. 2021. 793. doi: 10.1016/j.scitotenv.2021.148628
Download pdf
up
eISSN: 2221-6197 DOI: 10.31301/2221-6197