Biomics

The discharge of wastewater contaminated with industrial dyes into various reservoirs poses a significant threat to ecosystems and human health. The use of microorganisms capable of discoloring synthetic dyes is currently being considered an inexpensive, environmentally friendly and effective alternative to physico-chemical methods for cleaning colored wastewater. Bacteria of the genus Pseudomonas have an extremely active metabolism, which allows them to survive in reservoirs and soils polluted with various environmental pollutants. The purpose of this study is to evaluate the ability of bacterial strains of Pseudomonas sp. discolor triphenylmethane dye crystal purple. Three bacterial strains of Pseudomonas sp. 4 HM, 10 HM and 24 HM were isolated from soils contaminated with chemical waste, which were assigned to the genus Pseudomonas by analyzing the nucleotide sequence of the 16S rRNA gene. The ability of these bacteria to grow and decolorize crystalline violet at a concentration of 10-50 mg/l in solid and liquid nutrient media was investigated. It was shown that all three strains could grow and partially adsorb the dye on a solid nutrient medium in the entire studied concentration range, including in the presence of 3 mM nickel. In a liquid nutrient medium in the presence of 10 mg/l, all strains demonstrated a low level of decolorization: 36, 44, and 34%, for strains of Pseudomonas sp. 4HM, 10 HM, and 24 HM, respectively. At higher concentrations of the dye, inhibition of bacterial culture growth was observed.

Pseudomonas sp., synthetic dyes, crystalline violet, bioremediation, discoloration

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