Biomics

Experimenters sometimes need to amplify fairly long DNA fragments using PCR, which is not always possible under standard conditions. In such cases, long-range PCR is performed, the features of which are an extended elongation stage time of at least 1 minute for 1 kb length of template, a mixture of highly processive DNA polymerase and DNA polymerase with editing activity, allowing the removal of incorrectly inserted nucleotides capable of interrupting further polymerization, as well as the use of additional components in the reaction mixture, which are PCR enhancers. Various substances of chemical and biological nature act as such enhancers during the amplification of long DNA fragments. Despite the fact that the priorities for long-range PCR have changed somewhat over time, primarily due to the rapid development of high-throughput DNA sequencing of the latest generations and the possibility of long and ultra-long reads, nevertheless, the demand for amplification of long DNA fragments remains and even the range of their applications is expanding, including those beyond biological tasks.

PCR, long-range PCR, long templates, enhancer

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