Biomics

The article considers the problem of searching for associations of allelic variants of microsatellite loci with protein content in pea seeds. Among all leguminous crops characterized by high protein content, pea is the most adaptive crop to the conditions of the Urals steppe zone of the Republic of Bashkortostan. Its cultivation is promising in this territory. To identify allelic variants that can be used in the future as DNA markers of high content of reserve seed proteins (legumin, vicilin, convicilin), genotyping of varieties and lines of seed pea of different ecological and geographical origin was carried out. According to the Bradford method, the content of total protein in the studied cultivars was determined, and cultivars contrasting in this trait were identified. Two groups of samples with high (23.5±0.4 - 26.1±0.5%) and low (18.0±0.3 - 19.7±0.3%) protein content were formed. Then, genotyping of the above groups was performed by SSR analysis. As a result of the applied methodology, information on allelic composition of varietal samples with high and low content of spare seed proteins was obtained. The obtained data are planned to be used as additional criteria for selection of parental forms in crosses to obtain high-protein hybrids.

seed pea, Pisum sativum L., microsatellite analysis, seed protein content

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