Biomics

Eggplant (Solanum melongena L.) is an important vegetable crop from the Solanaceae family and one of the most popular vegetable crops in many countries around the world. Transformation of eggplant plants remains one of the main problems of plant genetic engineering due to the relatively low efficiency of callus genesis in different varieties of this crop. Our study analyzed the callus formation of two white-fruited eggplant varieties on a standard regeneration medium with the addition of different concentrations and combinations of hormones in 5 variants. The phytohormones used in the work were 1-naphthaleneacetic acid (NAA), kinetin, which is a cytokinin - a plant hormone that promotes cell division in the presence of auxin and is widely used for callus formation or to induce shoot growth, 6-benzylaminopurine (6-BAP), which is used to accelerate plant growth. Option I with 0.5 mg/l 6-BAP and 2.0 mg/l NAA, option II with 5.0 mg/l 6-BAP and 0.1 mg/l NAA, option III with 2.0 mg/l l 6-BAP and 0.1 mg/l NAA, option IV with 2.0 mg/l 6-BAP and 2.0 mg/l kinetin, option V and 10.0 mg/l 6-BAP and 0.2 mg/l NAA. The most suitable were option 2, where some calli formed growths in the form of roots, and in others the rudiments of microplants appeared, and option 3, where a very loose callus of white or light brown color was formed, on which the rudiments of microplants also appeared. But later, in both variants, all calli died. In variants 1, 4 and 5, the cotyledon explants turned brown, first at the edges, and then completely, and then died. Unfortunately, our study did not select optimal combinations of hormones that would be suitable for effective callusogenesis of different eggplant varieties and the work will continue.   

callusogenesis, Solanum melongena L., 6-BAP, NAA, in vitro

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