Biomics

Nicotiana tabacum L. is a plant of the Solanaceae family, which is an industrial cultivated species that can be used as biofactories for the production of drugs, vaccines or valuable small metabolites; tobacco plants also have a unique ability for rapid and efficient callusogenesis. Because of this, it can be used as a model plant cell system. Two varieties of smoking tobacco Virginia 202 and Yubileiny new 142 were considered, in which the efficiency of callusogenesis was studied under standard conditions on a medium with the addition of 1 mg/l 6- BAP (6-benzylaminopurine) and 0.1 mg/l NAA (naphthylacetic acid) with using regeneration through the callus stage. As a result, the variety Virginia 202 showed a high potential for callus formation, in addition, it adapted well in laboratory conditions after growth in vitro and produced mature seeds. This makes it possible to expand the range of varieties used with high callus formation efficiency for studying models with introduced genetic changes.

callus formation, Nicotiana tabacum L., 6-BAP, NUC, in vitro, Virginia 202, Jubilee new 142

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