Biomics

A study was conducted on 20-hydroxyecdysone at a concentration of 10-10 mol/l to 10-7 mol/l, fed in sugar syrup on the lifespan of workers under conditions of hyperthermia (50°C). The study was carried out both in summer and during wintering. Low concentrations of 20E up to 0.4×10-9 mol/l inclusive promoted the increase in resistance to hyperthermia, which, when increased to 2×10-9, sharply drops by 1.8 times and rises again at a concentration of 1×10-7 mol/l. It has been suggested that when exogenous 20E was supplied with food at a concentration of 2×10-9 mol/l, the enzymatic oxidation of 20E was activated, which causes a decrease in the level of endogenous 20E titer in the hemolymph and change in the sign of the effect. The change of bees survival time under hyperthermia with half-maximal effective concentration (EC50) of 20E 1×10-9 mol/l can be used as a method for determining the content of 20E. In this way it is determined that the Leuzea honey sample contains 2×10-5 mol/kg (10 mg/kg) 20E. The content of 20Е in Leuzea honey sample determined in this way was 2×10-5 mol/kg (10 mg/kg)

20-hydroxyecdysone, honey bee, hyperthermia, longevity, dose-effect, honey, Rhaponticum carthamoides (Willd.) Iljin

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