Year: 2022
Pages: 209-219
Number: Volume 14, issue 3
Type: scientific article
DOI: https://doi.org/10.31301/2221-6197.bmcs.2022-15
Topic: Article
Authors: Sokolnikova L.V., Bulmakova D.S., Nevzorova J.V., Suleimanova A.D.
Bacteria of the Pantoea genus have a positive effect on the growth and development of plants. In the genome of the P. brenneri strain3.5.2 we identified genes and genetic clusters associated with their multiple PGP-properties, previously confirmed by biochemical and microbiological methods. The strains are capable of hydrolyzing soil phytates and are active against inorganic soil phosphates: hydroxyapatite, phosphorite, and tricalcium phosphate. The gcd and pqqE genes responsible for the production of gluconic acid involved in the processes of phosphorus mobilization were identified. When grown on media with various sources of unavailable phosphorus, P. brenneri strains were able to secrete acid and alkaline phosphatases, the activity of which depended on the time of bacterial cultivation and the source of phosphate in the medium.
Pantoea brenneri, phosphates mobilization, acid phosphatase, alkaline phosphatase, phosphate-solubilizing bacteria
1. Algire M.A. Restrictionless cloning. Methods Enzymol. 2013. V. 529. P. 125-134. doi: https://doi.org/10.1016/B978-0-12-418687-3.00009-4
2. Alori E.T., Glick B.R., Babalola O.O. Microbial Phosphorus Solubilization and Its Potential for Use in Sustainable Agriculture. Front. Microbiol. 2017. V. 8. P. 1-8. doi: 10.3389/fmicb.2017.00971
3. Aziz R.K., Bartels D., Best A.A., <…>Vonstein V., Wilke A., Zagnitko O. The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008. V. 9. P. 75. doi: 10.1186/1471-2164-9-75
4. Cabugao K.G., Timm C.M., Carrell A.A., Childs J., Lu T.Y.S., Pelletier D.A., Norby R.J. Root and rhizosphere bacterial phosphatase activity varies with tree species and soil phosphorus availability in Puerto Rico tropical forest. Front. Plant Sci. 2017. V. 8. P. 1834. doi: 10.3389/fpls.2017.01834
5. Capek P., Kasanke C.P., Starke R., Zhao Q., Tahovska K. Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils // Biol. Fertil. Soils. 2021. V. 57(7). P. 991-1005. doi: 10.1007/s00374-021-01587-9
6. Chen C., Xin K., Liu H., Cheng J., Shen X., Wang Y., ZhangL. Pantoeaalhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat. Sci. Rep. 2017. V. 7. P. 41564. doi: 10.1038/srep41564
7. Chen W., Yang F., Zhang L., Wang J. Organic acid secretion and phosphate solubilizing efficiency of Pseudomonas sp. PSB12: effects of phosphorus forms and carbon sources. Geomicrobiol. J. 2015. V. 33 (10). P. 870-877. doi: 10.1080/01490451.2015.1123329
8. Dutkiewicz J., Mackiewicz B., Lemieszek M.K., Golec M., Milanowski J. Pantoea agglomerans: a mysterious bacterium of evil and good. Part Ⅳ. Beneficial effects. Ann. Agric. Environ. Med. 2016. V. 23(2). P. 206-207, 211-216. doi: 10.5604/12321966.1203879
9. Itkina D.L., Suleimanova A.D., Sharipova M.R. Pantoea brenneri AS3 and Bacillus ginsengihumiM2.11 as potential biocontrol and plant growth-promotion agents. Microbiology (Microbiologiya). 2021. V. 90(2). P. 210-218. doi: 10.1134/S0026261721020053
10. Fraser T.D., Lynch D.H., Entz M.H., Dunfield K.E. Quantification of bacterial non-specific acid (phoC) and alkaline (phoD) phosphatase genes in bulk and rhizosphere soil from organically managed soybean fields. Appl. Soil Ecol. 2017. V. 111. P. 48-56. doi: 10.1016/j.apsoil.2016.11.013
11. Han S.H., Kim C.H., Lee J.H., Park J.Y., Cho S.M., Park S.K. Inactivation of pqq genes of Enterobacter intermedium 60-2G reduces antifungal activity and induction of systemic resistance. FEMS Microbiol. Lett. 2008. V. 282(1). P. 140-146. doi: 10.1111/j.1574-6968.2008.01120.x
12. Kalayu G. Phosphate Solubilizing Microorganisms: Promising Approach as Biofertilizers. Plant Cell. 2019. V. 2(4). P. 6-9. doi: 10.1155/2019/4917256
13. Kumar R., Shastri B. Role of Phosphate-Solubilising Microorganisms in Sustainable Agricultural Development // Agro-Environ. Sustainability. 2017. P. 271-303. doi:10.1007/978-3-319-49724-2_13
14. Li J.T., Yang J., Chen D.C., Zhang X.L., Tang Z.S. An optimized mini-preparation method to obtain highquality genomic DNA from mature leaves of sunflower. Genet. Mol. Res. 2007. V. 6(4). P. 1064-1071.
15. Lidbury, I.D.E.A., Scanlan, D.J., Murphy, A.R.J. et al. A widely distributed phosphate-insensitive phosphatase presents aroute for rapid organophosphorus remineralization in the biosphere. Proc. Natl. Acad. Sci. USA. 2022. V. 119(5). e2118122119. doi: 10.1073/pnas.2118122119
16. Lu L., Chang M., Han X., Wang Q., Wang J., Yang H., Guan Q., Dai S. Beneficial effects of endophytic Pantoea ananatis with ability to promote rice growthunder saline stress. J. Gen. Appl. Microbiol. 2021. V. 131(4). P. 1-13. doi: 10.1111/jam.15082
17. Mei C., Chretien R.L., Amaradasa B.S., He Y., Turner A., Lowman S. Characterization of Phosphate Solubilizing Bacterial Endophytes and Plant Growth Promotion In Vitro and in Greenhouse. Microorganisms. 2021. V.9(9). P.1-11. doi: 10.3390/microorganisms9091935
18. Mira A., Klasson L., Andersson S.G.E. Microbial genome evolution: sources of variability. Curr. Opin. Microbiol. 2002. V. 5(5). P. 506-512. doi: 10.1016/s1369-5274(02)00358-2
19. Nascimento F.X., Hernandez A.G., Glick B.R., Rossi M.J. The extreme plant-growth-promoting properties of Pantoea phytobeneficialis MSR2 revealed by functional and genomic analysis. Environ. Microbiol. 2020. V. 22(4). P. 1341-1355. doi: 10.1111/1462-2920.14946
20. Olanrewaju O.S., Glick B.R., Babalola O.O. Mechanisms of action of plant growth promoting bacteria. World J. Microbiol. Biotechnol. 2017. V. 33(11). P. 197. doi: 10.1007/s11274-017-2364-9
21. Paredes-Paliz K.I., Pajuelo E., Doukkali B., Caviedes M.A., Rodriguez-Llorente I.D., Mateos-Naranjo E. Bacterial inoculants for enhanced seed germination of Spartina densiflora: Implications for restoration of metal polluted areas. Mar. Pollut. Bull. 2016. V. 110(1). P. 396-399. doi: 10.1111 / plb.12693
22. Perez E., Sulbaran M., Ball M.M., Yarzabal L.A. Isolation and characterization of mineral phosphatesolubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biol. Biochem. 2007. V.39(1). P. 2905-2914. doi:
10.1016/j.soilbio.2007.06.017
23. Rawat P., Das S., Shankhdhar D., Shankhdhar S.C. Phosphate-solubilizing microorganisms: mechanism and their role in phosphate solubilization and uptake. J. Soil Sci. Plant Nutr. 2021. V. 21(1). P. 49-68. doi: 10.1007/s42729-020-00342-7
24. Sakurai M., Wasaki J., Tomizawa Y., Shinano T., Osaki M. Analysis of bacterial communities on alkaline phosphatase genes in soil supplied with organic matter. J. Soil Sci. Plant Nutr. 2008. V. 54(1). P. 62-71. doi: 10.1111/j.1747-0765.2007.00210.x
25. Sambrook J., Fritsch E.R., Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Lab. Press. 1989. V. 2.
26. Sorty A.M., Meena K.K., Choudhary K., Bitla U.M., Minhas P.S., Krishnani K.K. Effect of Plant Growth Promoting Bacteria Associated with Halophytic Weed (Psoralea corylifolia L) on Germination and Seedling Growth of Wheat Under Saline Conditions. Appl. Biochem. Biotechnol. 2016. V. 180(5). P. 872-880. doi: 10.1007/s12010-016-2139-z
27. Suleimanova A.D., Beinhauer A., Valeeva L.R., Chastukhina I.B., Balaban N.P., Shakirov E.V., Greiner R., Sharipova M.R. Novel Glucose-1-Phosphatase with High Phytase Activity and Unusual Metal Ion Activation from Soil Bacterium Pantoea sp. Strain 3.5.1. Appl. Environ. Microbiol. 2015. V. 81(19). P. 6790-6799. doi: 10.1128/AEM.01384-15
28. Suleimanova A.D., Itkina D.L., Pudova D.S., Sharipova M.R. Identification of Pantoea phytatehydrolyzing rhizobacteria based on their phenotypic features and multilocus sequence analysis (MLSA). Microbiology (Microbiologiya). 2021. V. 90(1). P. 87-95. doi: 10.1134/S0026261721010112
29. Tabatabai M.A., Bremner J.M. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 1969. V. 1(4). P. 301-307. doi: 10.1016/0038-0717(69)90012-1
30. Tian J., Ge F., Zhang D., Deng S., Liu X. Roles of Phosphate Solubilizing Microorganisms from Managing Soil Phosphorus Deficiency to Mediating Biogeochemical P Cycle. Biology. 2021. V. 10(2). P. 1-19. doi: 10.3390/biology10020158
31. Vasseur-Coronado M., du Boulois H.D., Pertot I., Puopolo G. Selection of plant growth promoting rhizobacteria sharing suitable features to be commercially developed as biostimulant products. Microbiol. Res. 2021. V. 245. P. 126672. doi: 10.1016/j.micres.2020.126672
32. Xiao C.Q., Chi R.A., Huang X.H., Zhang W.X., Qiu G.Z., Wang D.Z. Optimization for rock phosphate solubilization by phosphate-solubilizing fungi isolated from phosphate mines. Ecol. Eng. 2008. V. 33(2). P. 187-193. doi: 10.1016/j.ecoleng.2008.04.001
33. Zhu J., Li M., Whelan M. Phosphorus activators contribute to legacy phosphorus availability in agricultural soils: A review. Sci. Total Environ. 2018. V. 612. P. 522-537. doi: 10.1016 /j.scitotenv.2017.08.095