Biomics

We studied the effect of 24-epibrassinolide (EB) on growth, proline content, the state of wheat seedling antioxidant system under water deficit, modeled by 12% polyethylene glycol. It was found that under drought EB-pretreatment has a protective effect on wheat plants, stabilizing the state of the antioxidant system, regulating the balance of hydrogen peroxide and peroxidase activity, as well as inducing the additional accumulation of proline, which was reflected in maintaining the higher level of mitotic activity in apical root cells of these plants.Задание 1

Triticum aestivum L., 24-epibrassinolide, water deficit, mitotic index, proline, hydrogen peroxide, peroxidase

1. Alam P., Albalawi T. H., Altalayan F. H.,. Bakht M.A., Ahanger M.A., Raja V., Ashraf M., Ahmad P. 24-epibrassinolide (EBR) confers tolerance against NaCl stress in soybean plants by up-regulating antioxidant system, ascorbate-glutathione cycle, and glyoxalase system. Biomolecules. 2019. V. 9. Article: 640. DOI: 10.3390/biom9110640

2. Allagulova Ch.R., Maslennikova D.R., Aval'baev A.M., Fedorova K.A., Yuldashev R.A., Shakirova F.M. Vliyanie 24-epibrassinolida na rost rastenii pshenitsy i soderzhanie degidrinov v usloviyakh kadmievogo stressa. Fiziologiya rastenii. 2015. T.62 (№ 4). S. 499-505. DOI: 10.7868/S0015330315040028 [Effect of 24-epibrassinolide on wheat plant growth and dehydrin content under cadmium stress] (In Russian)

3. Aval'baev A.M., Yuldashev R. A., Fatkhutdinova R. A., Urusov F. A, Safutdinova Yu. V., Shakirova F. M. Vliyanie 24 epibrassinolida na gormonal'nyi status rastenii pshenitsy pri deistvii khlorida natriya. Prikladnaya biokhimiya i mikrobiologiya. 2010. T. 46(1). S.109-112. [Effect of 24 epibrassinolide on the hormonal status of wheat plants under the action of sodium chloride] (In Russian)

4. Bates L.S., Waldran R.P., Teare I.D. Rapid determination of free proline for water stress studies. Plant Soil. 1973. V. 39. P. 205-208. DOI: 10.1007/bf00018060

5. Bezrukova M.V., Fatkhutdinova R.A., Shakirova F.M. Zashchitnoe deistvie agglyutinina zarodysha pshenitsy na protekanie mitoza v kornyakh prorostkov Triticum aestivum pri vozdeistvii kadmiya. Fiziologiya rastenii. 2016. T. 63 (№3). S. 382-389. DOI: 10.7868/S0015330316030027 [Protective effect of wheat germ agglutinin on the course of mitosis in the roots of Triticum aestivum seedlings under the influence of cadmium] (In Russian)

6. Bradford M.M. A rapid and sensitive methods for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 1976. P. 248-254. DOI: 10.1006/abio.1976.9999

7. Fabregas N., Lozano-Elena F., Blasco-Escamez D., Tohge T., Martinez-Andujar C., Albacete A., Osorio S., Bustamante M., Riechmann J. L., Nomura T., Yokota T., Conesa A., Afocea F.P., Fernie A.R., Cano-Delgado A.I. Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth. Nat. Commun. 2018. V. 9 (4680). DOI: 10.1038/s41467-018-06861-3

8. González-Garcı́a M.-P., Vilarrasa-Blasi J., Zhiponova M., Divol F., Mora Garcia S., Russinova E., CanoDelgado A. I. Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots. Development. 2011. V. 138. P. 849-859. DOI: 10.1242/dev.057331

9. Khairullin R.M., Yarullina L.G., Troshina N.B., Akhmetova I.E. Aktivatsiya khitooligosakharidami okisleniya ortofenilendiamina prorostkami pshenitsy v prisutstvii shchavelevoi kisloty. Biokhimiya. 2001. T. 66 (3). S. 354-358 [Activation by chitooligosaccharides of orthophenylenediamine oxidation by wheat seedlings in the presence of oxalic
acid] (In Russian)

10. Kishor P.B.K., Sangam S., Amrutha R.N., Laxmi P.S., Naidu K.R., Rao S., Rao S., Reddy K.J., Theriappan P., Sreenivasulu N. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implication in plant growth and abiotic stress tolerance. Current Sci. 2005. V. 88. P. 424-438

11. Laxa M., Liebthal M., Telman W., Chibani K., Dietz K. The role of the plant antioxidant system in drought tolerance. Antioxidants. 2019. V. 8 (94). DOI: 10.3390/antiox8040094

12. Lima J. V., Lobato A. K. S. Brassinosteroids improve photosystem II efficiency, gas exchange, antioxidant enzymes and growth of cowpea plants exposed to water deficit. Physiol. Mol. Biol. Plants. 2017. V. 23. P. 59-72. DOI: 10.1007/s12298-016-0410-y

13. Matysik J., Alia B.B., Mohanty P. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr. Sci. 2002. V. 82. P. 525-532.

14. Mhamdi A., Van Breusegem F. Reactive oxygen species in plant development. Development. 2018. V. 145 (dev164376). DOI: 10.1242/dev.164376

15. Miransari M., Smith D.L. Plant hormones and seed germination. Environ. Exp. Bot. 2014. V. 99. P. 110- 121. DOI :10.1016/j.envexpbot.2013.11.005

16. Mwadzingeni L., Shimelis H., Tesfay S., Tsilo T.J. Screening of bread wheat genotypes for drought tolerance using phenotypic and proline analyses. Front. Plant Sci. 2016. V. 7 (1276). DOI: 10.3389/fpls.2016.01276

17. Osmolovskaya N., Shumilina J., Kim A., Didio A., Grishina T., Bilova T. , Keltsieva O.A. , Zhukov V., Tikhonovich I., Tarakhovskaya E., Frolov A., Wessjohann L.A. Methodology of drought stress research: experimental setup and physiological characterization. Int. J. Mol. Sci. 2018. V. 19 (4089). DOI: 10.3390/ijms19124089

18. Planas-Riverola A., Gupta A ., Betegón-Putze I ., Bosch N ., IbañesM., Caño-Delgado A.I. Brassinosteroid signaling in plant development and adaptation to stress. Development. 2019. V. 146 (dev151894). DOI:10.1242/dev.151894.

19. Segal L.M., Wilson R.A. Reactive oxygen species metabolism and plant-fungal interactions. Fungal Genet. Biol. 2018. V. 110. P. 1-9. DOI: 10.1016/j.fgb.2017.12.003

20. Sripinyowanich S., Klomsakul P., Boonburapong B., Bangyeekhun T., Asami T., Gu H., Buaboocha T., Chadchawan S. Exogenous ABA induces salt tolerance in indica rice (Oryza sativa L.): The role of OsP5CS1 and OsP5CR gene expression during salt stress. Environ. Exp. Bot. 2013. V. 86. P. 94-105. DOI: 10.1016/j.envexpbot.2010.01.009

21. Szabados L., Savoute A. Proline: a multifunctional aminoacid. Trends Plant Sci. 2010. V. 15 (2). P. 89- 97. DOI: 10.1016/j.tplants.2009.11

22. Tunc-Ozdemir M., Jones A. M. BRL3 and AtRGS1 cooperate to fine tune growth inhibition and ROS activation. PLoS ONE. 2017. V. 12 (e0177400). DOI: 10.1371/journal.pone.0177400

23. Vlasova T.A. Malyi praktikum po fiziologii rastenii. M.: Izd-vo MGU. 1994. 183 s. [Small workshop on plant physiology] (In Russian)

24. Wani A.S., Ahmad A. Hayat Sh., Tahir I. Epibrassinolide and proline alleviate the photosynthetic and yield inhibition under salt stress by acting on antioxidant system in mustard. Plant Physiol. Biochem. 2019. V. 135. P.385-394. DOI:
10.1016/j.plaphy.2019.01.002