eISSN: 2221-6197 DOI: 10.31301/2221-6197

Biomics

Archive

Indexing
  1. Biomics
  2. →
  3. 2020
  4. →
  5. Volume 12, no. 4
  6. →
  7. Effect of 20-Hydroxyecdysone and Sadenosylmethionine on the ecdysone receptor gene EcR transcriptional activity in housefly Musca domestica L.

Effect of 20-Hydroxyecdysone and Sadenosylmethionine on the ecdysone receptor gene EcR transcriptional activity in housefly Musca domestica L.

Year: 2020

Pages: 480-491

Number: Volume 12, issue 4

DOI: https://doi.org/10.31301/2221-6197.bmcs.2020-42

Topic: Article

Authors: Nikonorov Yu.M., Akhmetkireeva T.T.!, Benkovskaya G.V.

Download pdf

Summary:

Steroid hormone 20-hydroxyecdysone (20E) initiates larval molting start and metamorphosis and regulates reproduction. Its basic receptor is heterodimer including proteins EcR and USP. Ecdysone receptor gene EcR coding protein EcR is a key regulatory element of gene circuits cover considerable part of genes, implicated in growth and development as well as in reproduction of progeny and reactions of organisms to unfavorable factors of environment. The source of methyl groups S-adenosylmethionine (SAM) is in use for biosynthesis of juvenile hormone (JH), methylation of histone proteins and DNA. The main aim of our investigation was evaluation of transcriptional activity of housefly Musca domestica ecdysone receptor gene EcR under adding into ration of 20E and SAM in non-lethal concentrations. Experiments were carried out with larvae and adults of housefly from laboratory strains Shgen and Lgen differ in life span of adults. Change of gene EcR transcripts content in common pool of mRNA in the cells of muscles and gonads, as well as DNA methylation level in 5’-terminal site registered by quantitative real time PCR (RT-PCR). The results of our investigations allow us to suggest existence of mechanism for regulating expression of the EcR gene in M. domestica which is sensitive to exogenic20E and heat stress action as well as to presence of SAM in food. Variations in the mRNA quantitative ratios of EcR gene 5’-and 3’-terminal regions depending on tissue type, gender and age support the hypothesis that this gene can encode several isoforms of the protein EcR. The detected changes in the status of DNA methylation in the 5'-terminal region of the gene and fluctuations in the representation of different mRNA sites after SAM processing suggest the involvement of DNA methylation/demethylation processes in the regulation of EcR gene expression in M. domestica.

Keywords:

Ecdysone receptor; Musca domestica; 20-hydroxyecdysone; S-adenosylmethionine; DNA methylation

References:

  1. Akhmetkireeva T.T., Benkovskaya G.V., Vasyl’ev A.G. Izmenchivost' formy i razmerov kryla v selektirovannyh po prodolzhitel'nosti zhizni liniyah Musca domestica L.: geometricheskaya morfometriya. [Variability of size and shape of wings in longevityselected strains of house fly (Musca domestica L.): geometric morphometrics]. Ecol. Genetics. 2018. V.16(1). S.35-44. doi: 10.17816/ecogen 16135-44 (In Russian).
  2. Andres A.J., Fletcher J.C., Karim F.D., Thummel C.S. Molecular analysis of the initiation of insect metamorphosis: a comparative study of Drosophila ecdysteroid-regulated transcription. Dev. Biol. 1993. V.160(2). P. 388-404. doi: 10.1006/dbio.1993.1315
  3. Benkovskaya G.V. Opportunities and limitations of changes in lifespan in laboratory experiment. Adv. Gerontol. 2011. V.1(3). P.255-259. doi: 10.1134/S2079057011030039
  4. Benkovskaya G.V. Principy soderzhaniya laboratornyh linij nasekomyh [Principles of maintaining the laboratory strains of insects]. Biomics. 2017. 9(1). S. 24-32. (In Russian).
  5. Bonasio R., Li Q., Lian J., Mutti N.S., Jin L., Zhao H., Zhang P., Wen P., Xiang H., Ding Y., Jin Z., Shen S.S., Wang Z., Wang W., Wang J., Berger S.L., Liebig J., Zhang G., Reinberg D. Genome-wide and caste-specific DNA methylomes of the ants Camponotus floridanus and Harpegnathos saltator. Curr. Biol. 2012. V.22(19). P. 1755-1764. doi: 10.1016/j.cub.2012.07.042
  6. Codd V., Dolezel D., Stehlik J., Piccin A., Garner K.J., Racey A.N., Straatman K.R., Louis E.J., Costa R., Sauman I., Kyriacou C.P., Rosato E. Circadian Rhythm Gene Regulation in the Housefly Musca domestica. Genetics. 2007. V.177. P.1539–1551. doi: 10.1534/genetics.107.079160
  7. Fang F., Xu Y., Jones D., Jones G. Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling. FEBS J. 2005. 272(7). P.1577-1589. doi:10.1111/j.1742- 4658.2005.04578.x
  8. Jindra M., Malone F., Hiruma K., Riddiford L.M. Developmental profiles and ecdysteroid regulation of the mRNAs for two ecdysone receptor isoforms in the epidermis and wings of the tobacco hornworm, Manduca sexta. Dev Biol. 1996. V.180(1). P.258-272. doi:10.1006/dbio.1996.0299
  9. Jindra M., Palli S.R., Riddiford L.M. The juvenile hormone signaling pathway in insect development. Annu. Rev. Entomol. 2013. V.58. P.181–204. doi:10.1146/annurev-ento-120811-153700
  10. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001. V.25(4). P.402-408. doi: 10.1006/meth.2001.1262
  11. Mouillet J.F., Henrich V.C., Lezzi M., Vogtli M. V. Differential control of gene activity by isoforms A, B1 and B2 of the Drosophila ecdysone receptor. Eur. J. Biochem. 2001. V.268(6). P.1811–1819. doi: 10.1046/j.1432-1033.2001.02051.x
  12. Nikonorov Yu.M., Akhmetkireeva T.T., Benkovskaya G.V. Metilirovanie DNK u Musca domestica L. [DNA methylation in house fly Musca domestica L.]. Biomics. 2016. 8(3). S. 266-274. (In Russian).
  13. Nikonorov Yu.M., Akhmetkireeva T.T., Benkovskaya G.V. Ontogeneticheskie i transgeneracionnye effekty izbytka donorov metil'nyh grupp v laboratornyh liniyah komnatnoj muhi. [Developmental transgenerational effects of methyl group donors redundancy in the laboratory strains of house fly]. Biomics. 2017. 9(2). S. 141-147. (In Russian).
  14. Odinokov V.N., Galyatdinov I.V., Nedopekin D.V., Khalilov L.M., Shashkov A.S., Kachala V.V., Dinan L., Lafont R.. Phytoecdysteroids from the juice of Serratula coronata L. (Asteraceae). Insect Biochem. Mol. Biol. 2002. V.32. P. 161-165. doi: 10.1016/S0965- 1748(01)00106-0
  15. Rauschenbach I.Y., Lukashina N.S., Maksimovsky L.F., Korochkin L.I. Stress-like reaction of Drosophila to adverse environmental factors. J. Comp. Physiol. 1987. V.157. P. 519-531. doi: 10.1007/BF00691837
  16. Riddiford L.M. How does juvenile hormone control insect metamorphosis and reproduction? Gen. Comp. Endocrinol. 2012. V.179(3). P. 477-484. doi: 10.1016/j.ygcen.2012.06.001
  17. Riddiford L. M., Cherbas P., Truman J. W. Ecdysone receptors and their biological actions. Vitam. Horm. 2000. V.60. P.1-73. doi: 10.1016/s0083- 6729(00)60016-x
  18. Russel S., Ahsburner M. Ecdysone-regulated chromosome puffing in Drosophila melanogaster. In: Metamorphosis: postembryonic reprogramming of gene expression in amphibian and insect cells. Eds. Gilbert L.I., Tata J.R., Atkinson B.G. San Diego, Academic Press. 1996. P.109-173. doi:10.1016/B978-012283245- 1/50005-1
  19. Schwedes C., Tulsiani S., Carney G. Ecdysone receptor expression and activity in Drosophila melanogaster. J. Insect Physiol. 2011. V.57. P. 899-907. doi: 10.1016/j.jinsphys.2011. 03.027
  20. Siegenthaler C., Maroy P., Hediger M., Dübendorfer A., Bopp D. Hormones and sex-specific transcription factors jointly control yolk protein synthesis in Musca domestica. Intern. J. Evol. Biol. 2009. ID 291236. doi: 10.4061/2009/201236
  21. Simon A.F., Shih C., Mack A., Benzer S. Steroid Control of Longevity in Drosophila melanogaster. Science. 2003. V.299(5611). P. 1407–1410. doi:10.1126/science.1080539
  22. Soller M., Bownes M., Kubli E. Control of oocyte maturation in sexually mature Drosophila females. Dev. Biol. 1999. V.208. P.337-351. doi: 10.1006/dbio.1999.9210
  23. Talbot W.S., Swyrid E.A., Hogness D.S. Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms. Cell. 1993. V.73(7). P. 1323-1337. doi: 10.1016/0092-8674(93)90359-x
  24. Thomas H.E., Stunnenberg H.G., Stewart A.F. Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle. Nature. 1993. V.362(6419). P. 471-475. doi: 10.1038/362471a0
  25. Toyota K., Miyakawa H., Hiruta C., Furuta K., Ogino Y., Shinoda T., Tatarazako N., Miyagawa S., Shaw J.R., Iguchi T. Methyl farnesoate synthesis is necessary for the environmental sex determination in the water flea Daphnia pulex. J. Insect Physiol. 2015. V.80. P. 22–30. doi: 10.1016/j.jinsphys.2015.02.002
  26. Watanabe T., Takeuchi H., Kubo T. Structural diversity and evolution of the N-terminal isoformspecific region of ecdysone receptor-A and -B1 isoforms in insects. BMC Evol. Biol. 2010. V.10(40). doi: 10.1186/1471-2148-10-40
Download pdf
up
eISSN: 2221-6197 DOI: 10.31301/2221-6197