Biomics

The effect of the composition of the nutrient medium on the production of the phytohormone indolyl-3-acetic acid and carotene during the cultivation of the epiphytic strain Pantoea sp. IB-BF was studied. The level of auxin and carotene in the culture fluid of the proposed variants of nutrient media varied from 2 to 21 μg/ml and from 0.06 to 0.172 OD₄₆₁/mg dry biomass, respectively. A significant effect of the presence of a protein component (tryptone, peptone or yeast extract) on the biosynthesis of auxin was noted. The secretion of carotene increased considerably under conditions of low nitrogen content and high C:N ratio (potato-glucose medium). In the presence of 100 μg/ml tryptophan, a reliable increase in the IAA yield was recorded by 18% compared to the auxin level on the medium without tryptophan. Synthesis of indole-3-acetic acid and carotene, successful colonization of wheat roots with the Pantoea sp. IB-BF with an increase in the number KOE of more than 10⁴, were carried out under conditions of an abiotic load similar to the presence of sodium chloride of at least 100 μM in the medium.

PGPR-bacteria, phytohormones, indolyl-3-acetic acid, Pantoea, carotenes, biologics

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