eISSN: 2221-6197 DOI: 10.31301/2221-6197

Chemical mutagenesis of Taraxacum brevicorniculatum with sodium azide

Year: 2020

Pages: 211-217

Number: Volume 12, issue 2

Type: scientific article

Summary:

Taraxacum brevicorniculatum and Taraxacum kok-saghyz are promising rubber-bearing dandelion species. T. brevicorniculatum, in contrast to kok-saghyz, is characterized by high parameters of vegetative growth, high seed productivity, but it accumulates very little natural rubber in the roots. The aim of the study was to carry out of chemical mutagenesis of T. brevicorniculatum using sodium azide and to determine the rubber content in the roots of the mutant plants. The use of 1 mM sodium azide solution had no significant effect on rubber accumulation in the roots and on leaf growth. The highest parameters of leaf growth were typical for plants obtained after treatment with 10 mM sodium azide, while two plants with a high content of rubber in the roots were obtained. When 20 mM sodium azide was used, a median lethal dose of the mutagen was achieved and the highest results in rubber accumulation were obtained - more than 5% per dry root mass, which is more than twice as much as in control plants and close to that of the kok-saghyz. The mutant forms of T. brevicorniculatum obtained in the course of work can be used in further breeding in order to select fast-growing and highly productive rubber-bearing varieties of this species.

Keywords:

Taraxacum brevicorniculatum, natural rubber, chemical mutagenesis, sodium azide

References:

  1. Baimuhametova E.A., Lashtabova S.V., Golovina V.Y., Kimsanbaev O.H., Kuluev B.R. Application of induced mutagenesis for increasing the genetic polymorphism of cotton. Biomics. 2017. V. 9. No.4. P. 370–379.
  2. Chaldayeva D.A., Khusainov A.D. The use of natural and synthetic rubber in the production of tires. Bulletin of the Technological University. 2013. V. 16. No. 11. P. 195–198. (In Russian).
  3. Dykman A. S., Busygin V.M., Moiseyev I.I., Gilmanov K.K., Fedortsova E.V. Global tendencies in production of natural and isoprene rubber. Economics and Management. V. 1. P. 46–52. (In Russian).
  4. Garshin M. V., Kartuha A. I., Kuluev R. Taraxacum kok-saghyz: cultivation features and perspectives of introduction to modern production. Biomics. 2016. V. 8. No. 4. P. 323–333. (In Russian)
  5. Ilin M. M., Yakimov A. Kauchukonosy i guttaperchenosy SSSR [Rubber and gutta-percha plants of the USSR]. In: Rastitel'noye syrye SSSR. Sbornik statey. Vol. 1. Tekhnicheskiye rasteniya. Moscow; Leningrad. 1950. P. 61–141. (In Russian)
  6. Ilyin M.M. Kauchukonosnost' flory SSSR [The rubberiness of the flora of the USSR]. In Rubber and Rubber-bearing plants. 1953. V. 2. Ed. Ilina M.M. P. 9–104. (In Russian)
  7. Khairullin R.Z., Vlasova Yu.S., Yanov V.V. Perspektivy ispol'zovaniya al'ternativnykh vidov rastitel'nogo syr'ya dlya proizvodstva natural'nogo kauchuka [Prospects for the use of alternative types of plant materials for the production of natural rubber]. Bulletin of the Technological University. 2014. V. 17 (13). P. 205–206. (In Russian)
  8. Kirschner J., Štěpánek J., Černý T., De Heer P., van Dijk P.J. Available exsitu germplasm of the potential rubber crop Taraxacum kok-saghyz belongs to a poor rubber producer, brevicorniculatum (Compositae–Crepidinae). Genetic Resources and Crop Evolution. 2012. V. 60 (2). P. 455–471.
  9. Kuluev B. R., Garafutdinov R. R., Maksimov I. V., Sagitov A. M., Chemeris D. A., Knyazev A. V., Vershinina Z. R., Baymiev An. K., Muldashev A. A., Baymiev Al. K., Chemeris V. Natural rubber, its sources and components. Biomics. 2015. V. 7(4). P. 224–283. (In Russian)
  10. Kuluev B. R., Muldashev A. A., Minchenkov N. D., Chemeris A. V. Searching for potential rubber-bearing plants in the flora of the Republic of Bashkortostan. Rastitelnye resursy. 2019. V. 55. No. 3. P. 317–333.
  11. Polovenko I. S., Filippov D. I., Pravdin F. N., Furman M. Kok-sagyz [Kok-saghyz]. 1950. Moscow. 167 p. (In Russian).
  12. Ramirez-Cadavid D., Cornish K., Michel F. Taraxacum kok-saghyz: compositional analysis of a feedstock for natural rubber and other products. Ind. Crop. Prod. 2017. V. 107. P. 624–640. doi: 10.1016/j.indcrop.2017.05.043
  13. Shi, Z., Chen, Y. L., Chen, Y. S., Lin, Y. R., Liu, S. W., Ge, X. J., Gao, T. G., Zhu, S. X., Liu, Y., Yang, Q. E., Humphries, C. J., Raab-Straube, E. von, Gilbert, M. G., Nordenstam, B., Kilian, N., Brouillet, L., Illarionova, I. D., Hind, D. J. N., Jeffrey, C., Bayer, R. J., Kirschner, J., Greuter, W., Anderberg, A. A., Semple, J. C., Štěpánek, J., Freire, S. E., Martins, L., Koyama, H., Kawahara, T., Vincent, L., Sukhorukov, A. P., Mavrodiev, E. V. & Gottschlich, G. / Wu, Z. Y., Raven, P. H. & Hong, D. Y., eds. 2011. Asteraceae (Compositae). Flora of China Volume 20-21 (Asteraceae). Science Press (Beijing) & Missouri Botanical Garden Press (St. Louis). 2011. 894 p.
  14. Spanò D., Pintus F., Mascia C., Scorciapino M.A., Casu M., Floris G., Medda R. Extraction and characterization of a natural rubber from Euphorbia characias Biopolymers. 2012. V. 97. P. 589–594. doi: 10.1002/bip.22044.
  15. Stepanov G.R. Perspektivy kul'tury krym-sagyza [Perspectives of the krym-saghyz culture]. Kauchuk I Rezina. 1940. No.8. P. 67–69. (In Russian).
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eISSN: 2221-6197 DOI: 10.31301/2221-6197