Biomics

A vector construct based on pJN105 carrying the pseudophytochelatin pph6 gene was created, and rhizobia strains with pph6 gene were obtained. Inoculation with transformed strains positively affected the biomass of plants under the conditions of toxic effects of cadmium, despite a decrease in the number of nodules formed on the roots. The experiments showed that it is advisable to use the pph6 gene transformation to increase the resistance of both rhizobia and legume-rhizobial symbiosis to the effects of cadmium for the purpose of their further use for phytoremediation.

heavy metals, cadmium, phytoremediation, Rhizobium leguminosarum, rhizobia, phytochelatin, pseudophytochelatin

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