Biomics

The aim of this work was to assess the influence of the ethylene precursor 1-aminocyclopropanoic acid (ACC) on the level, distribution between shoot and root, and auxin metabolism in plants of Arabidopsis (Arabidopsis thaliana) of the original Columbia ecotype. Exogenous ACC reduced the content of IAA in the roots, but did not affect the accumulation of their mass. We previously showed that the loss of sensitivity to ethylene in Arabidopsis plants both as a result of a mutation (etr1-1) and during the pharmacological action of an ethylene receptor inhibitor 1-MCP on plants transformed with DR5::GUS led to an increase in the level of IAA in the roots. All this allows us to conclude that ethylene is able to prevent the excessive accumulation of auxins in the roots, thereby supporting their growth. The introduction of ACC into the medium led to a decrease in the ratio of IAA content in the roots and shoots against the background of a constant total IAA content in these plant organs, as well as to an increase in the expression of the WES1 gene in the roots, which is responsible for binding of auxins to amino acids. It can be assumed that the possible mechanisms for reducing the level of IAA in the roots under the influence of ethylene are an increase in the outflow of auxin from shoots to roots and an increase in its inactivation by conjugation.

Arabidopsis thaliana; ACC; IAA; root mass; IAA allocation; IAA conjugation; WES1

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