Biomics

The paper presents the results of the study of the effect of ABA on distribution of auxins and morphogenesis in vitro in ABA deficient barley mutant AZ34 and its parental genotype Steptoe. The linkage between the effect of ABA on morphogenesis in vitro and auxin transport as well as the role of peroxidases in the action of ABA on morphogenesis in vitro was studied. It was established that reduced level of ABA in the callus of Az34 mutant was accompanied by a decline in activity of peroxidases resulting, inter alia, in a delay in the embryoid development. ABA is suggested to participate in the implementation of the processes coupled with auxin transport although it is concluded that mechanism of ABA action on morphogenesis in vitro is more complicated. We conclude that ABA is necessary for normal passage of morphogenesis in vitro, including embryoidogenesis.

barley, ABA, auxins, peroxidases, morphogenesis in vitro

1. Cho M., Cho H.-T. The function of ABCB transporters in auxin transport. Plant Signal Behav. 2013. V. 8(2). e22990. DOI: 10.4161/psb.22990
2. Fischer C., Speth V., Fleig-Eberenz S., Neuhaus G. Induction of zygotic polyembryos in wheat: Influence of auxin polar transport. Plant Cell. 1997. V. 9(10). P. 1767–1780. DOI: 10.1105/tpc.9.10.1767
3. Kruglova N.N., Egorova O.V., Seldimirova O.A., Zajcev D.Yu., Zinatullina A.E. Svetovoj mikroskop kak instrument v biotehnologii rastenij. Ufa: Gilem, Bashk. jencikl., 2013. 128 s. [Light microscope as a tool in plant biotechnology]. (In Russian).
4. Marjamaa K., Kukkola E.M., Fagerstedt K.V. The role of xylem class III peroxidases in lignification. J Exp Bot. 2009. V. 60(2). P. 367–376. DOI: 10.1093/jxb/ern278
5. Miao Y., Lv D., Wang P., Wang X.C., Chen J., Miao C., Song C.P. An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress response. Plant Cell. 2006. V. 18(10). P. 2749–2766. DOI: 10.1105/tpc.106.044230
6. Seldimirova O.A., Kruglova N.N., Veselov D.S., Janovskaja A.A. Optimizacija sostava pitatel'noj sredy dlja indukcii kallusoobrazovanija u jachmenja sorta Steptoe i ego ABK-deficitnogo mutanta AZ3. Biomics. 2017. V. 9(4). S. 298–303. [Optimization of the nutrient medium composition for induction of callus formation in barley cv. Steptoe and its ABA-deficient mutant AZ34]. (In Russian).
7. Seldimirova O.A., Kudoyarova G.R., Kruglova N.N., Galin I.R., Veselov D.S. Somatic Embryogenesis in Wheat and Barley Calli in vitro Is Determined by the Level of Indoleacetic and Abscisic Acids. Russian Journal of Developmental Biology. 2019. V. 50(3). P. 124–135. DOI: 10.1134/S1062360419030056
8. Titova G.E., Seldimirova O.A., Kruglova N.N., Galin I.R., Batygina T.B. Phenomenon of “Siamese Embryos” in Cereals in vivo and in vitro: Cleavage Polyembryony and Fasciations. Russian Journal of Developmental Biology. 2016. V. 47(3). P. 122–137. DOI: 10.1134/S10623604160300x61
9. Skoog F., Miller C.O. Chemical regulation of growth and organ formation in plant tissue cultured in vitro. Symp. Soc. Exp. Biol. 1957. V. 11. P. 118–131.
10. Tretyakova I.N., Park M.E., Kazachenko A.S., Shuklina A.S., Kudoyarova G.R., Akhiyarova G.R., Korobova A.V., Veselov S.U. Сontent and immunohistochemical localization of hormones during in vitro somatic embryogenesis in long-term proliferating Larix sibirica cultures. Plant Cell, Tiss. Org. Cult. 2019. V. 136(3). P. 511-522. DOI: 10.1007/s11240-018-01533-y
11. Troshina N.B. Perekis' vodoroda kak reguljator ustojchivosti rastenij i kallusov pshenicy k gribnym patogenam: avtoreferat dis. ... doktora biologicheskih nauk. Vseros. nauch.-issled. in-t rastenievodstva im. N.I. Vavilova RASHN: SPb, 2007. 42 s. [Hydrogen peroxide as a regulator of the resistance of plants and callus of wheat to fungal pathogens]. (In Russian).
12. Tsukagoshi H., Busch W., Benfey P.N. Transcriptional regulation of ROS controls transition from proliferation to differentiation in the root. Cell. 2010. V. 143(4). P. 606–616. DOI: 10.1016/j.cell.2010.10.020.
13. Xia X.J., Zhou Y.H., Shi K., Zhou J., Foyer C.H., Yu J.Q. Interplay between reactive oxygen species and hormones in the control of plant development and stress tolerance. J Exp Bot. 2015. V. 66(10). P. 2839–2856. DOI: 10.1093/jxb/erv089
14. Yao C., Finlayson S.A. Abscisic acid is a general negative regulator of Arabidopsis axillary bud growth. Plant Physiol. 2015. V. 169(1). P. 611–626. DOI: 10.1104/pp.15.00682