Biomics

Expansins are proteins that provide plant growth by stimulating the divergence of cellulose microfibrils during cell expansion. We obtained and analyzed 6 lines of hairy roots of Nicotiana tabacum with constitutive expression of the NtEXPA5 tobacco expansin gene, which were characterized by higher growth rates and increased productivity under normal conditions, compared with the control. Hairy roots of tobacco overexpressing the NtEXPA5 gene were characterized by increased tolerance to NaCl, mannitol, cadmium acetate, and CuSO₄. Based on the obtained data, the binary vector pCambia 1301 with the NtEXPA5 gene can be proposed for creating hairy roots with increased growth parameters and productivity under both normal and stress conditions. The prospect of using of hairy roots as a model object for rapid testing of targeted binary vectors is discussed.

expansins, hairy roots, salinity, water deficit, heavy metals, cadmium, copper, growth, stress tolerance, Nicotiana tabacum, Agrobacterium rhizogenes

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