eISSN: 2221-6197 DOI: 10.31301/2221-6197

Some novelties in CRISPR/Cas genome editing and related areas

Year: 2019

Pages: 315-343

Number: Volume 11, issue 3

Type: scientific article

Summary:

The ongoing research on CRISPR/Cas loci is so diverse, that can be called no fewer than “The CRISPR/Cas World” or even “The CRISPR/Cas Universe”. This review covers only a small part of existing data, with an emphasis on plant organisms. There are briefly described new methods for different stages of genome editing and for its various options such as knockout editing (KO), knockin editing (KI), editing of nitrogenous bases in DNA (ABE and CBE) and in RNA (RBE), prime editing (PE). The use of CRISPR/Cas systems for other purposes, including highly sensitive detection of specific nucleic acid fragments, both with and without amplification methods, is discussed.

Keywords:

genome editing, CRISPR/Cas system, CRISPR/Cas technology, CRISPR/Cas components, guide RNA, ant-CRISPR

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