Biomics

Currently, it is known that the honey stomach of a honeybee contains a large number of probiotic bacteria with a broad spectrum of antimicrobial activity against pathogenic for bees and humans microorganisms [Olofsson et al., 2014]. The number and microflora species composition of the honey stomach depends on the season, the source and amount of nectar, the health of bees and the presence of other microorganisms in the collected nectar [Butler et al., 2013]. The problem of winter easing and death of bee colonies in many countries, including Russia, is still extremely relevant. The northern populations of the dark forest bee subspecies are more resistant to pathogenic microorganisms. We assume that the specificity of the microbiome in populations of the dark forest bee and the closely related social immunity of bee colonies play an important role in this. We have obtained isolated colonies of microorganisms from the worker bee honey stomachs of the Bashkir population of A. mellifera mellifera L., determined the motility, the ratio to the Gram stain, catalase activity and the fermentation of carbohydrates of microorganisms from these colonies. Pure bacterial cultures isolated from the honey stomach of healthy worker bees by morphological, tinctorial and biochemical characteristics are representatives of the genus Lactobacillus, divided by the selectivity of carbohydrate fermentation into 4 groups. The general contamination of honey stomach with microorganisms is significantly lower than that of mature honey, which confirms the antagonism of the useful stomach microflora with respect to extraneous microbiota.

probiotics, lactic acid bacteria, dark forest bee, honey stomach

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