Biomics

This work is devoted to the study of morphological and anatomical structures of callus tissues obtained during the cultivation of Schisandra chinensis (Turc.) Baill.) аnd Eleuterococcus senticosus Maxim.) оn nutrient media in in vitro conditions, which opens the possibility for obtaining BAS and obtaining microsprouts through morpho- and gemmogenesis. For the production of S. chinensis callus tissue, parts of the leaf blade, the internode of the microspore and the lower part of the mother shoot were used; E. senticosus - the lower part of the microsprout. In order to induce callusogenesis, an in vitro cultivation method was used on various types of nutrient media (Murashige-Skoog and Quoirin-Lepoivre), including media containing different kinds of growth regulators: 6-benzylaminopurine at a concentration of 1.0 mg/L, indolylbutyric acid at a concentration of 0.1 mg/L, and thidiazuron at a concentration of 0.1 mg/L. Morphological evaluation of callus tissue was made, then callus sections were made, on the basis of which microscope slide were prepared. They were given an anatomical evaluation of the tissue. S. chinensis managed to obtain three types of callus tissues from different parts of the plant, differing in color, friability, morphogenicity, presence of bud of gemmogenesis. In E. senticosus we received only one type of callus tissue. Callus tissue from the internode of S. chinensis proved to be the most promising for cultivation for the purpose of obtaining plant biomass. The resulting S. chinensis and E. senticosus calli were passivated on a nutrient medium in order to induce structural morphogenesis. With microscopy of callus tissue from the S. chinensis leaf blade, a dense, compact structure of the callus tissue was revealed, as well as elements of the conducting system near the focus of formation of structural gemmogenesis. The callus tissue from the internode of the S. chinensis microsprout had large cells having a regular structure; it was possible to detect a well-formed microbud, as well as the structure of the conducting system. Callus tissue from the part of E. senticosus microscope showed the presence of chaotically located, unconnected large colorless cells.

Schisandra chinensis, Eleuterococcus senticosus, callus tissue, structural morphogenesis, gemmogenesis, in vitro culture, microsprout

1. Akhmetova A.Sh. Razmnozhenie limonnika kitaiskogo (Schisandra chinensis (Turcz) Baill.) in vitro. Agrokhimiya. 2014. No. 11. P. 52-57. [Reproduction of Schisandra chinensis (Turcz) Baill.) in vitro - In Russian]
2. Murashige T.A., Skoog F. Revised medium for rapid growth and bio assays with tobacco tissue cultures // Physiol. Plant. 1962. V. 15. Р. 473–497.
3. Muratova S.A. Perfection of the method of clonal micropropagation of actinidia and magnolia vine of Chinese / SA Muratova and others // Modern gardening. Michurinsk, 2010. V.1 P. 96-100 [In Russian].
4. Nosov A.M. Use of cellular technologies for industrial production of biologically active substances of plant origin. Biotechnology, 2010. V.5 - P. 8-28. [In Russian].
5. Tut' E.A. Peculiarities of micropropagation of actinidia and magnolia vine magnesium. Agricultural Biology. 2008. No 3. P. 96-101 [In Russian].
6. Shornikov D.G. Development of a method for clonal multiplication of eleutherococcus senticosus (Rupr. et Maxim.) Maxim.). Biology of plant cells in vitro and biotechnology: Tez. IX Intern. Conf., Zvenigorod, September 8-12, 2008 - Moscow: ID FBK PRESS, 2008. P. 442. [In Russian].
7. Shornikov D.G. Perfection of the technology of reproduction of rare garden plants in culture in vitro and evaluation of their potential for resistance to abiotic stressors. Michurinsk.: Abstract, 2008. 27 P. [In Russian].
8. Upadyshev M.T.. Tut’ E.A. Specifics of propagation of actinidia and magnolia vine in in vitro and green cuttings. Fruit growing and grapes breeding of Russia. 2004. V. 11. P. 200-209. [In Russian].