Biomics

The three-day sterile wheat seedlings of Saratovskaya strain 29 were inoculated with A. brasilense Sp245 (endophytic strain) and Sp7 (epiphytic strain) with subsequent co-cultivation for 4 weeks to study the dependence of phenol oxidases activity in the presence of host plant. The inoculation of wheat with bacteria resulted in increase of laccase and Mn-peroxidase activity in the growing medium compared to the control samples. The obtained data indicate that Mn-peroxidases and laccases are accumulated in the bacterial cluster zones on the root surface activity thus indicating the possible role of these enzymes for azospirilles adaptation to rhizosphere conditions. It can be assumed that extracellular phenol oxidase production is functionally significant both for bacteria and host plants. The kinetic properties of laccases, lignin and Mn-peroxidases mediate the elimination of the negative effect of toxic substances accumulated in soil.

Azospirillum; phenol oxidase; biodegradation; remasol reactive blue R 19

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