Biomics

An hour after the introduction of exogenous zeatin into the nutrient solution of wheat plants, the content of cytokinins increased by 30 compared to the control, which is 5 times higher than the level of accumulation of hormones in plants incubated on the zeatin ribozide solution of the same molarity. The difference in the rate of accumulation of exogenous hormones was associated not with the rate of their absorption or decay, but with the features of the distribution (the predominant accumulation of zeatin in the cells, and its riboside - in the apoplast [Korobova et al., 2013], where cytokinins are more available to destruction with cytokinin oxidase. Inhibition of the secondary active transmembrane transport sharply reduced the content of zeatin in the root cells, but the effect of this treatment on the zeatin riboside distribution was less pronounced. Thus, there is a lesser dependence of processes of zeatin riboside cell absorption and its retention in cells on the presence of proton gradient as compared with zeatin. This may be important for the performance of transport function by zeatin riboside.

Triticum durum; cytokinins; zeatin; zeatin riboside; membrane transport; protonophore; immunohistochemical localization

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