Biomics

Essential oil rose is one of the world’s best known aromatic plants. Its products are widely used in the perfumery, cosmetics and food industry, as well as in medicine. The purpose of this work was to study the influence of cultivar, culture medium composition and term of cultivation on rooting during the process of clonal micropropagation of essential oil rose. In investigation we studied the cultivars Raduga, Lany, Lada, Michurinka, Festivalnaya, obtained with the participation of species Rosa damascena Mill., R. gallica L., R. alba L. Rooting of the shoots, formed after microcutting at the 2^nd stage of micropropagation in meristeme cuture, was carried out in vitro on the modified MS medium supplemented with auxins (NAA, IAA, IBA). It was found that for maximum frequency of rhizogenesis and the number of root formation ½MS medium with 0.5 mg L^-1 NAA was used. The highest frequency of rooting (100.0%) and the number of roots (7.3-7.7 pcs /shoot) were observed for Raduga and Festivalnaya cultivars. ‘Lada’ had the lowest frequency of rhizogenesis (85.7%), while ‘Lany’ had the lowest number of roots (4.9 pcs/shoot). It was shown that for Raduga cultivar, when using microcutting obtained in the 2^nd, 6^th, and 9^th passages, there were no significant differences in the frequency of rooting and the number of roots. At the same time, as the passage increased, a growth in the frequency of rhizogenesis and the number of roots on the explant was observed for ‘Michurinka’.

essential oil rose, clonal micropropagation, rooting, in vitro

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