Biomics

Jasmine tobacco Nicotiana alata is an ornamental plant with beautiful, but not large flowers. Therefore, to increase the decorative appeal of this plant is actual increase the size of its flowers. To this end, we carried out Agrobacterium-mediated transformation of N. alata leaf discs with a genetic engineering construction containing the ARGOS-LIKE gene of Arabidopsis thaliana and the dahlia mosaic virus promoter. We generated two lines of transgenic jasmine tobacco plants. One of the lines of transgenic plants was characterized by an increase in the length of the leaves by 21% and the stem height by 10% compared to the wild type. Another line of transgenic plants was characterized by an increase in the size of flowers: flower diameter by 13% and flower length by 8%, compared to wild type. Thus, in the course of the work we have shown the possibility of generating of transgenic N. alata plants by Agrobacterium-mediated transformation of leaf discs. The ARGOS-LIKE gene with the dahlia mosaic virus promoter can be recommended for the modification of flower sizes for jasmine tobacco.

Nicotiana alata, jasmine tobacco, ARGOS, dahlia mosaic virus promoter, organ size, flower, transgenic plants

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